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Title: The Relationship Between Anti-Ascaridia Galli Antibody Levels in the Egg Yolk and Number of Excreta Worm Eggs by ELISA Test
Contributor(s): Dao, Thi Hiep  (author); Hunt, Peter W (author); Hine, Brad (author); Swick, Robert A  (author)orcid ; Ruhnke, Isabelle  (author)orcid 
Publication Date: 2018
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Abstract: Ascaridia galli is one of the most abundant nematode parasites of poultry. This study aimed at determining the relationship between the levels of anti-A. galli in the egg yolks and number of worm eggs in the hens' excreta, which can then be used to develop a new method to detect A. galli infection in layer hens. The procedures to optimize the performance of enzyme-linked immunosorbent assay (ELISA) for detection of anti-A. galli antibodies in egg yolks were also examined. The results showed that blocking buffer with BSA (99%) diluted at 0.5%, secondary antibodies diluted at 1/15,000 and egg yolk samples diluted at 1/3,000 and 1/12,000 were optimal to maximize the performance of ELISA assay. Although anti-A. galli antibody levels in the egg yolk was not highly correlated with worm egg counts, ELISA test is still a potential method to detect A. galli infection in laying hens.
Publication Type: Journal Article
Source of Publication: Journal of Animal Husbandry Sciences and Technics, v.235, p. 84-89
Publisher: Animal Husbandry Association of Vietnam
Place of Publication: Vietnam
ISSN: 1859-476X
Field of Research (FOR): 070205 Animal Protection (Pests and Pathogens)
070708 Veterinary Parasitology
Socio-Economic Outcome Codes: 830309 Poultry
839901 Animal Welfare
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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