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Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/23582
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dc.contributor.authorDao, Thi Hiepen
dc.contributor.authorHunt, Peter Wen
dc.contributor.authorHine, Braden
dc.contributor.authorSwick, Robert Aen
dc.contributor.authorRuhnke, Isabelleen
dc.date.accessioned2018-08-29T10:55:00Z-
dc.date.issued2018-
dc.identifier.citationJournal of Animal Husbandry Sciences and Technics, v.235, p. 84-89en
dc.identifier.issn1859-476Xen
dc.identifier.urihttps://hdl.handle.net/1959.11/23582-
dc.description.abstractAscaridia galli is one of the most abundant nematode parasites of poultry. This study aimed at determining the relationship between the levels of anti-A. galli in the egg yolks and number of worm eggs in the hens' excreta, which can then be used to develop a new method to detect A. galli infection in layer hens. The procedures to optimize the performance of enzyme-linked immunosorbent assay (ELISA) for detection of anti-A. galli antibodies in egg yolks were also examined. The results showed that blocking buffer with BSA (99%) diluted at 0.5%, secondary antibodies diluted at 1/15,000 and egg yolk samples diluted at 1/3,000 and 1/12,000 were optimal to maximize the performance of ELISA assay. Although anti-A. galli antibody levels in the egg yolk was not highly correlated with worm egg counts, ELISA test is still a potential method to detect A. galli infection in laying hens.en
dc.languageenen
dc.publisherAnimal Husbandry Association of Vietnamen
dc.relation.ispartofJournal of Animal Husbandry Sciences and Technicsen
dc.titleThe Relationship Between Anti-Ascaridia Galli Antibody Levels in the Egg Yolk and Number of Excreta Worm Eggs by ELISA Testen
dc.typeJournal Articleen
dc.subject.keywordsAnimal Protection (Pests and Pathogens)en
dc.subject.keywordsVeterinary Parasitologyen
local.contributor.firstnameThi Hiepen
local.contributor.firstnamePeter Wen
local.contributor.firstnameBraden
local.contributor.firstnameRobert Aen
local.contributor.firstnameIsabelleen
local.subject.for2008070205 Animal Protection (Pests and Pathogens)en
local.subject.for2008070708 Veterinary Parasitologyen
local.subject.seo2008830309 Poultryen
local.subject.seo2008839901 Animal Welfareen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailtdao@myune.edu.auen
local.profile.emailpeter.hunt@csiro.auen
local.profile.emailbrad.hine@csiro.auen
local.profile.emailrswick@une.edu.auen
local.profile.emailiruhnke@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20180824-214738en
local.publisher.placeVietnamen
local.format.startpage84en
local.format.endpage89en
local.peerreviewedYesen
local.identifier.volume235en
local.contributor.lastnameDaoen
local.contributor.lastnameHunten
local.contributor.lastnameHineen
local.contributor.lastnameSwicken
local.contributor.lastnameRuhnkeen
dc.identifier.staffune-id:tdaoen
dc.identifier.staffune-id:rswicken
dc.identifier.staffune-id:iruhnkeen
local.profile.orcid0000-0003-3376-1677en
local.profile.orcid0000-0001-5423-9306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:23764en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleThe Relationship Between Anti-Ascaridia Galli Antibody Levels in the Egg Yolk and Number of Excreta Worm Eggs by ELISA Testen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorDao, Thi Hiepen
local.search.authorHunt, Peter Wen
local.search.authorHine, Braden
local.search.authorSwick, Robert Aen
local.search.authorRuhnke, Isabelleen
local.uneassociationUnknownen
local.year.published2018en
local.subject.for2020300304 Animal protection (incl. pests and pathogens)en
local.subject.for2020300909 Veterinary parasitologyen
local.subject.seo2020100411 Poultryen
local.subject.seo2020109902 Animal welfareen
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School of Environmental and Rural Science
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