Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/8205
Title: Proteomic assessment of host-associated microevolution in the fungus 'Thielaviopsis basicola'
Contributor(s): Coumans-Moens, Joelle  (author)orcid ; Harvey, John (author); Backhouse, David  (author)orcid ; Plojak, A (author); Raftery, M J (author); Nehl, David (author); Katz, Margaret E  (author); Pereg, Lily  (author)
Publication Date: 2011
DOI: 10.1111/j.1462-2920.2010.02358.x
Handle Link: https://hdl.handle.net/1959.11/8205
Abstract: 'Thielaviopsis basicola', a soil-borne pathogen with a broad host range and a cosmopolitan distribution, is emerging as a major risk to sustainable cotton production in Australia. Previous studies suggested that host specialization has occurred making 'T. basicola' an ideal model for a comparative proteomic analysis of strains isolated from different hosts. Elucidation of the genomic diversity and investigation of the functional differences in the Australian population could provide valuable information towards disease control. In this study, isolates of 'T. basicola' were investigated for genomic (internal transcribed spacers region), proteomic and cotton virulence level variations. Internal transcribed spacers sequence analysis revealed that isolates are grouped based on host of origin irrespective of geographical origin. At the proteome level a degree of diversity was apparent and hierarchical clustering analysis of the data also demonstrated a close correlation between the proteome and the host of origin. LC-MS/MS analysis and identification using cross-species similarity searching and de novo sequencing of host-specific differentially expressed proteins and the virulence-correlated proteome allowed successful identification of 43 spots. The majority were found to be involved in metabolism. Spots that were correlated with host and virulence differences included a hypothetical protein with a Rossman-fold NAD(P)(+)-binding protein domain, glyceraldehyde-3-phosphate dehydrogenase, arginase and tetrahydroxynaphthalene reductase.
Publication Type: Journal Article
Source of Publication: Environmental Microbiology, 13(3), p. 576-588
Publisher: Wiley-Blackwell Publishing Ltd
Place of Publication: United Kingdom
ISSN: 1462-2920
1462-2912
Fields of Research (FoR) 2008: 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)
060704 Plant Pathology
060409 Molecular Evolution
Socio-Economic Objective (SEO) 2008: 820301 Cotton
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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