Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/20480
Title: Formation of Hydrogen Sulfide from Cysteine in 'Saccharomyces cerevisiae' BY4742: Genome Wide Screen Reveals a Central Role of the Vacuole
Contributor(s): Winter, Gal  (author)orcid ; Cordente, Antonio G (author); Curtin, Chris (author)
Publication Date: 2014
Open Access: Yes
DOI: 10.1371/journal.pone.0113869Open Access Link
Handle Link: https://hdl.handle.net/1959.11/20480
Abstract: Discoveries on the toxic effects of cysteine accumulation and, particularly, recent findings on the many physiological roles of one of the products of cysteine catabolism, hydrogen sulfide (H₂S), are highlighting the importance of this amino acid and sulfur metabolism in a range of cellular activities. It is also highlighting how little we know about this critical part of cellular metabolism. In the work described here, a genome-wide screen using a deletion collection of 'Saccharomyces cerevisiae' revealed a surprising set of genes associated with this process. In addition, the yeast vacuole, not previously associated with cysteine catabolism, emerged as an important compartment for cysteine degradation. Most prominent among the vacuole-related mutants were those involved in vacuole acidification; we identified each of the eight subunits of a vacuole acidification sub-complex (V₁ of the yeast V-ATPase) as essential for cysteine degradation. Other functions identified included translation, RNA processing, folate-derived one-carbon metabolism, and mitochondrial iron-sulfur homeostasis. This work identified for the first time cellular factors affecting the fundamental process of cysteine catabolism. Results obtained significantly contribute to the understanding of this process and may provide insight into the underlying cause of cysteine accumulation and H₂S generation in eukaryotes.
Publication Type: Journal Article
Source of Publication: PLoS One, 9(12), p. 1-20
Publisher: Public Library of Science
Place of Publication: United States of America
ISSN: 1932-6203
Fields of Research (FoR) 2008: 060104 Cell Metabolism
Fields of Research (FoR) 2020: 310103 Cell metabolism
Socio-Economic Objective (SEO) 2008: 970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objective (SEO) 2020: 280102 Expanding knowledge in the biological sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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