Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/20478
Title: Metabolic Engineering of 'Pseudomonas putida' KT2440 for the Production of 'para'-Hydroxy Benzoic Acid
Contributor(s): Yu, Shiqin (author); Plan, Manuel R (author); Winter, Gal  (author)orcid ; Kromer, Jens O (author)
Publication Date: 2016
Open Access: Yes
DOI: 10.3389/fbioe.2016.00090Open Access Link
Handle Link: https://hdl.handle.net/1959.11/20478
Abstract: 'para'-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common preservatives in food, drugs, and personal care products, as well as high-performance bioplastics such as liquid crystal polymers. 'Pseudomonas putida' KT2440 was engineered to produce PHBA from glucose via the shikimate pathway intermediate chorismate. To obtain the PHBA production strain, chorismate lyase UbiC from 'Escherichia coli' and a feedback resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase encoded by gene 'aroG'D146N were overexpressed individually and simultaneously. In addition, genes related to product degradation (pobA) or competing for the precursor chorismate ('pheA' and 'trpE') were deleted from the genome. To further improve PHBA production, the glucose metabolism repressor 'hexR' was knocked out in order to increase erythrose 4-phosphate and NADPH supply. The best strain achieved a maximum titer of 1.73 g L⁻¹and a carbon yield of 18.1% (C-mol C-mol⁻¹) in a non-optimized fed-batch fermentation. This is to date the highest PHBA concentration produced by 'P. putida' using a chorismate lyase.
Publication Type: Journal Article
Source of Publication: Frontiers in Bioengineering and Biotechnology, v.4, p. 1-10
Publisher: Frontiers Research Foundation
Place of Publication: Switzerland
ISSN: 2296-4185
Fields of Research (FoR) 2008: 060104 Cell Metabolism
060113 Synthetic Biology
Fields of Research (FoR) 2020: 310103 Cell metabolism
310113 Synthetic biology
Socio-Economic Objective (SEO) 2008: 970106 Expanding Knowledge in the Biological Sciences
Socio-Economic Objective (SEO) 2020: 280102 Expanding knowledge in the biological sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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