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Title: Evaluating a novel application of optical fibre evanescent field absorbance: Rapid measurement of red 'colour' in winegrape homogenates
Contributor(s): Lye, Peter (author)orcid ; Bradbury, Ronald (author); Lamb, David (author)
Publication Date: 2013
DOI: 10.1117/12.2019633
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Abstract: Silica optical fibres were used to measure colour (mg anthocyanin/g fresh berry weight) in samples of red wine grape homogenates via optical Fibre Evanescent Field Absorbance (FEFA). Colour measurements from 126 samples of grape homogenate were compared against the standard industry spectrophotometric reference method that involves chemical extraction and subsequent optical absorption measurements of clarified samples at 520 nm. FEFA absorbance on homogenates at 520 nm (FEFA520h) was correlated with the industry reference method measurements of colour (R² = 0.46, n = 126). Using a simple regression equation colour could be predicted with a standard error of cross-validation (SECV) of 0.21 mg/g, with a range of 0.6 to 2.2 mg anthocyanin/g and a standard deviation of 0.33 mg/g. With a Ratio of Performance Deviation (RPD) of 1.6, the technique when utilizing only a single detection wavelength, is not robust enough to apply in a diagnostic sense, however the results do demonstrate the potential of the FEFA method as a fast and low-cost assay of colour in homogenized samples.
Publication Type: Conference Publication
Conference Name: PIAGENG 2013: Image Processing and Photonics for Agricultural Engineering, Sanya, China, 27th - 28th January, 2013
Source of Publication: Proceedings of PIAGENG 2013: Image Processing and Photonics for Agricultural Engineering, p. 87610R-1-87610R-6
Publisher: SPIE: International Society for Optical Engineering
Place of Publication: Bellingham, United States of America
ISSN: 0277-786X
Field of Research (FOR): 020504 Photonics, Optoelectronics and Optical Communications
030106 Quality Assurance, Chemometrics, Traceability and Metrological Chemistry
030107 Sensor Technology (Chemical aspects)
039903 Industrial Chemistry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
Series Name: SPIE Proceedings
Series Number : 8761
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Appears in Collections:Conference Publication
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