Cryptic species complexes in manipulative echinostomatid trematodes: when two become six

Abstract

Recent studies have shown that some digenean trematodes previously identified as single species due to the lack of distinguishing morphological characteristics actually consist of a number of genetically distinct cryptic species. We obtained mitochondrial 16S and nuclear ITS1 sequences for the redial stages of 'Acanthoparyphium' sp. and 'Curtuteria australis' collected from snails and whelks at various locations around Otago Peninsula, New Zealand. These two echinostomes are well-known host manipulators whose impact extends to the entire intertidal community. Using phylogenetic analyses, we found that 'Acanthoparyphium' sp. is actually composed of at least 4 genetically distinct species, and that a cryptic species of 'Curtuteria' occurs in addition to 'C. australis'. Molecular data obtained for metacercariae dissected from cockle second intermediate hosts matched sequences obtained for 'Acanthoparyphium' sp. A and 'C. australis' rediae, respectively, but no other species. The various cryptic species of both 'Acanthoparyphium' and 'Curtuteria' also showed an extremely localized pattern of distribution: some species were either absent or very rare in Otago Harbour, but reached far higher prevalence in nearby sheltered inlets. This small-scale spatial segregation is unexpected as shorebird definitive hosts can disperse trematode eggs across wide geographical areas, which should result in a homogeneous mixing of the species on small geographical scales. Possible explanations for this spatial segregation of the species include sampling artefacts, local adaptation by first intermediate hosts, environmental conditions, and site fidelity of the definitive hosts.