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https://hdl.handle.net/1959.11/8065
Title: | Reduction of the in vitro pro-inflammatory response by macrophages to poly(3-hydroxybutyrate-co-3-hydroxyvalerate) | Contributor(s): | Wu, ACK (author); Grondahl, L (author); Jack, KS (author); Foo, MX (author); Trau, M (author); Hume, DA (author); Cassady, Alan (author) | Publication Date: | 2006 | DOI: | 10.1016/j.biomaterials.2006.05.010 | Handle Link: | https://hdl.handle.net/1959.11/8065 | Abstract: | This study evaluates the pro-inflammatory response to the thermoplastic biopolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) through the analysis of cellular responses in vitro. The murine macrophage RAW264.7 cell line was cultured on solvent cast PHBV films, which was found to induce pro-inflammatory activity that required direct contact between the material and the macrophages. The identity of the pro-inflammatory stimulus was determined by culturing bone marrow-derived macrophages from bacterial lipopolysaccharide (LPS) hyporesponsive C3H/HeJ mice and CpG non-responsive TLR9–/– mice on PHBV. The lack of a pro-inflammatory response by the C3H/HeJ cells indicates that the pro-inflammatory agent present within PHBV is predominately LPS while the TLR9–/– macrophages confirmed that CpG-containing bacterial DNA is unlikely to contribute to the activity. A series of purification procedures was evaluated and one procedure was developed that utilized hydrogen peroxide treatment in solution. The optimized purification was found to substantially reduce the pro-inflammatory response to PHBV without adversely affecting either the molecular structure or molecular weight of the material thereby rendering it more amenable for use as a biomaterial in vivo. | Publication Type: | Journal Article | Source of Publication: | Biomaterials, 27(27), p. 4715-4725 | Publisher: | Elsevier BV | Place of Publication: | Netherlands | ISSN: | 1878-5905 0142-9612 |
Fields of Research (FoR) 2008: | 090304 Medical Devices | Socio-Economic Objective (SEO) 2008: | 970111 Expanding Knowledge in the Medical and Health Sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article School of Science and Technology |
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