Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/8065
Title: Reduction of the in vitro pro-inflammatory response by macrophages to poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Contributor(s): Wu, ACK (author); Grondahl, L (author); Jack, KS (author); Foo, MX (author); Trau, M (author); Hume, DA (author); Cassady, Alan  (author)
Publication Date: 2006
DOI: 10.1016/j.biomaterials.2006.05.010
Handle Link: https://hdl.handle.net/1959.11/8065
Abstract: This study evaluates the pro-inflammatory response to the thermoplastic biopolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) through the analysis of cellular responses in vitro. The murine macrophage RAW264.7 cell line was cultured on solvent cast PHBV films, which was found to induce pro-inflammatory activity that required direct contact between the material and the macrophages. The identity of the pro-inflammatory stimulus was determined by culturing bone marrow-derived macrophages from bacterial lipopolysaccharide (LPS) hyporesponsive C3H/HeJ mice and CpG non-responsive TLR9–/– mice on PHBV. The lack of a pro-inflammatory response by the C3H/HeJ cells indicates that the pro-inflammatory agent present within PHBV is predominately LPS while the TLR9–/– macrophages confirmed that CpG-containing bacterial DNA is unlikely to contribute to the activity. A series of purification procedures was evaluated and one procedure was developed that utilized hydrogen peroxide treatment in solution. The optimized purification was found to substantially reduce the pro-inflammatory response to PHBV without adversely affecting either the molecular structure or molecular weight of the material thereby rendering it more amenable for use as a biomaterial in vivo.
Publication Type: Journal Article
Source of Publication: Biomaterials, 27(27), p. 4715-4725
Publisher: Elsevier BV
Place of Publication: Netherlands
ISSN: 1878-5905
0142-9612
Fields of Research (FoR) 2008: 090304 Medical Devices
Socio-Economic Objective (SEO) 2008: 970111 Expanding Knowledge in the Medical and Health Sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Science and Technology

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