Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/4837
Title: Micropropagation of juvenile tissue of Eucalyptus erythronema x eucalyptus stricklandii cv. 'urrbrae gem'
Contributor(s): Glocke, P (author); Delaporte, K (author); Collins, G (author); Sedgley, Margaret  (author)
Publication Date: 2006
DOI: 10.1079/IVP2005740
Handle Link: https://hdl.handle.net/1959.11/4837
Abstract: Micropropagation via enhanced axillary shoot proliferation was investigated in the ornamental Eucalyptus cv. 'Urrbrae Gem' using in vitro germinated seedlings and was successfully achieved using woody plant medium (WPM) supplemented with 2.2 μM benzylaminopurine, 1.0 μM α-naphthaleneacetic acid, and 1.5 μM gibberellic acid (GA₃), gelled with 5 g l−1 Phytagel®. Shoot proliferation was greater on WPM and QL media with GA3 compared to B5, AP, and TK media with or without GA3. GA3 was required for shoot elongation as the internodes were otherwise very short and unsuitable for multiplication or root initiation. Root initiation was improved using (1/2) WPM supplemented with 20 μM indole-3-butyric acid (IBA) over a 7 d pulse, followed by subculture to IBA-free medium, compared to placing shoots on low levels of IBA for 4–6 wk. Plantlets were successfully hardened off to the natural environment via a fogger at 67% relative, humidity at 21°C for 3 d and continued to thrive as potted plants. This is the first report of successful, micropropagation in an ornamental eucalypt (subgenus Symphyomyrtus) from seedling explants.
Publication Type: Journal Article
Source of Publication: In Vitro Cellular and Developmental Biology - Plant, 42(2), p. 139-143
Publisher: CABI
Place of Publication: Germany
ISSN: 1475-2689
1054-5476
Fields of Research (FoR) 2008: 070602 Horticultural Crop Improvement (Selection and Breeding)
Socio-Economic Objective (SEO) 2008: 820209 Ornamentals, Natives, Flowers and Nursery Plants
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Science and Technology

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