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https://hdl.handle.net/1959.11/48215
Title: | Distinct metabolism of apolipoproteins (a) and B-100 within plasma lipoprotein(a) |
Contributor(s): | Diffenderfer, Margaret R (author); Lamon-Fava, Stefania (author); Marcovina, Santica M (author); Barrett, P Hugh R (author) ; Lel, Julian (author); Dolnikowski, Gregory G (author); Berglund, Lars (author); Schaefer, Ernst J (author) |
Publication Date: | 2016-04 |
Early Online Version: | 2015-11-06 |
DOI: | 10.1016/j.metabol.2015.10.031 |
Handle Link: | https://hdl.handle.net/1959.11/48215 |
Abstract: | | Objectives
Lipoprotein(a) [Lp(a)] is mainly similar in composition to LDL, but differs in having apolipoprotein (apo) (a) covalently linked to apoB-100. Our purpose was to examine the individual metabolism of apo(a) and apoB-100 within plasma Lp(a).
Materials and Methods
The kinetics of apo(a) and apoB-100 in plasma Lp(a) were assessed in four men with dyslipidemia [Lp(a) concentration: 8.9–124.7 nmol/L]. All subjects received a primed constant infusion of [5,5,5-2H3] L-leucine while in the constantly fed state. Lp(a) was immunoprecipitated directly from whole plasma; apo(a) and apoB-100 were separated by gel electrophoresis; and isotopic enrichment was determined by gas chromatography/mass spectrometry.
Results
Multicompartmental modeling analysis indicated that the median fractional catabolic rates of apo(a) and apoB-100 within Lp(a) were significantly different at 0.104 and 0.263 pools/day, respectively (P = 0.04). The median Lp(a) apo(a) production rate at 0.248 nmol/kg · day− 1 was significantly lower than that of Lp(a) apoB-100 at 0.514 nmol/kg · day− 1 (P = 0.03).
Conclusion
Our data indicate that apo(a) has a plasma residence time (11 days) that is more than twice as long as that of apoB-100 (4 days) within Lp(a), supporting the concept that apo(a) and apoB-100 within plasma Lp(a) are not catabolized from the bloodstream as a unit in humans in the fed state.
Publication Type: | Journal Article |
Source of Publication: | Metabolism, 65(4), p. 381-390 |
Publisher: | Elsevier Inc |
Place of Publication: | United States of America |
ISSN: | 1532-8600 0026-0495 |
Fields of Research (FoR) 2020: | 320101 Cardiology (incl. cardiovascular diseases) |
Socio-Economic Objective (SEO) 2020: | 200105 Treatment of human diseases and conditions |
Peer Reviewed: | Yes |
HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
Appears in Collections: | Journal Article
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