Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/21282
Title: | Porcine Epidemic Diarrhea Virus RNA Present in Commercial Spray-Dried Porcine Plasma Is Not Infectious to Naive Pigs | Contributor(s): | Opriessnig, Tanja (author); Xiao, Chao-Ting (author); Gerber, Priscilla Freitas (author) ; Zhang, Jianqiang (author); Halbur, Patrick G (author) | Publication Date: | 2014 | Open Access: | Yes | DOI: | 10.1371/journal.pone.0104766 | Handle Link: | https://hdl.handle.net/1959.11/21282 | Abstract: | Porcine epidemic diarrhea virus emerged in North America in April 2013 and has since been identified in 30 U.S. States, Canada and Mexico. The rapid spread of PEDV has raised concerns about the role of feed and particularly pork-by-product components such as spray-dried porcine plasma (SDPP) in PEDV transmission. The aim of this study was to determine the infectivity of PEDV RNA present in commercial SDPP. Specifically, 40 3-week-old PEDV naïve pigs were randomly assigned to one of five treatment groups. At day post inoculation (dpi) 0, NEG-CONTROL pigs were sham-inoculated, PEDV-CONTROL pigs received cell culture propagated PEDV, and SDPP-CONTROL pigs were switched to a diet with 5% SDPP containing 5.160.1 log10 PEDV RNA copies/g. To evaluate a potential positive effect of anti-PEDV antibodies in SDPP on PEDV challenge, four days prior to PEDV challenge the pigs in the SDPP-PEDV group were switched to and remained on a 5% SDPP diet through dpi 28. Another group, EGG-PEDV, was orally administered a commercial egg-derived liquid PEDV globulin product from dpi -4 through 6. All PEDV-CONTROL pigs began shedding PEDV in feces by dpi 3 and seroconverted between dpi 7 and 14, whereas pigs in NEG-CONTROL and SDPP-CONTROL groups remained PEDV RNA negative and did not seroconvert to PEDV for the study duration. This indicates no evidence of infectivity of the PEDV RNA in the SDPP lot utilized. Furthermore, under the study conditions SDPP or egg-derived liquid PEDV globulin addition did not significantly alter PEDV-shedding or overall disease course after experimental challenge. | Publication Type: | Journal Article | Source of Publication: | PLoS One, 9(8), p. 1-10 | Publisher: | Public Library of Science | Place of Publication: | United States of America | ISSN: | 1932-6203 | Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070706 Veterinary Medicine 070704 Veterinary Epidemiology |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300907 Veterinary medicine (excl. urology) 300905 Veterinary epidemiology |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
---|---|
Appears in Collections: | Journal Article |
Files in This Item:
File | Description | Size | Format |
---|
SCOPUSTM
Citations
63
checked on Sep 14, 2024
Page view(s)
1,142
checked on Sep 15, 2024
Download(s)
2
checked on Sep 15, 2024
Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.