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https://hdl.handle.net/1959.11/21198
Title: | Monitoring Autophagy in Yeast using FM 4‐64 Fluorescence | Contributor(s): | Journo, Dikla (author); Winter, Gal (author)![]() |
Publication Date: | 2008 | DOI: | 10.1016/s0076-6879(08)03207-2 | Handle Link: | https://hdl.handle.net/1959.11/21198 | Abstract: | The original observations and experiments dealing with autophagy were purely morphological in nature. Even though more and more molecular techniques have been introduced, experimenters are often asked to provide visual evidence of autophagic processes in order to back up data obtained via other means. In yeast as well, autophagosomes were initially defined morphologically and indirectly, by observing intravacuolar autophagic bodies that accumulate upon starvation. This can be achieved by electron microscopy, which affords very high resolution but is time consuming and costly, or by light microscopy, which is a relatively inaccurate method of scoring autophagy. A third alternative, which we present here, is to use the unique properties of the fluorescent dye FM 4-64 to follow the accumulation of autophagic bodies. | Publication Type: | Book Chapter | Source of Publication: | Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, p. 79-88 | Publisher: | Academic Press | Place of Publication: | Amsterdam, Netherlands | ISBN: | 9780123745484 | Fields of Research (FoR) 2008: | 060104 Cell Metabolism | Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences | HERDC Category Description: | B1 Chapter in a Scholarly Book | Publisher/associated links: | http://trove.nla.gov.au/version/43697610 | Series Name: | Methods in Enzymology | Series Number : | 451 | Editor: | Editor(s): Daniel J Klionsky |
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Appears in Collections: | Book Chapter |
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