Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/21188
Title: Aup1p, a Yeast Mitochondrial Protein Phosphatase Homolog, Is Required for Efficient Stationary Phase Mitophagy and Cell Survival
Contributor(s): Tal, Ruth (author); Winter, Gal  (author)orcid ; Ecker, Nitai (author); Klionsky, Daniel J (author); Abeliovich, Hagai (author)
Publication Date: 2006
DOI: 10.1074/jbc.m605940200
Handle Link: https://hdl.handle.net/1959.11/21188
Abstract: Autophagy is a catabolic membrane-trafficking process that occurs in all eukaryotic cells and leads to the hydrolytic degradation of cytosolic material in the vacuolar or lysosomal lumen. Mitophagy, a selective form of autophagy targeting mitochondria, is poorly understood at present. Several recent reports suggest that mitophagy is a selective process that targets damaged mitochondria, whereas other studies imply a role for mitophagy in cell death processes. In a screen for protein phosphatase homologs that functionally interact with the autophagy-dedicated protein kinase Atg1p in yeast, we have identified Aup1p, encoded by 'Saccharomyces cerevisiae' reading frame 'YCR079w'. Aup1p is highly similar to a family of protein phosphatase homologs in animal cells that are predicted to localize to mitochondria based on sequence analysis. Interestingly, we found that Aup1p localizes to the mitochondrial intermembrane space and is required for efficient mitophagy in stationary phase cells. Viability studies demonstrate that Aup1p is required for efficient survival of cells in prolonged stationary phase cultures, implying a pro-survival role for mitophagy under our working conditions. Our data suggest that Aup1p may be part of a signal transduction mechanism that marks mitochondria for sequestration into autophagosomes.
Publication Type: Journal Article
Source of Publication: Journal of Biological Chemistry, 282(8), p. 5617-5624
Publisher: American Society for Biochemistry and Molecular Biology
Place of Publication: United States of America
ISSN: 1083-351X
0021-9258
Fields of Research (FoR) 2008: 060108 Protein Trafficking
Socio-Economic Objective (SEO) 2008: 970106 Expanding Knowledge in the Biological Sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article

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