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https://hdl.handle.net/1959.11/20078
Title: | Role of HxkC, a mitochondrial hexokinase-like protein, in fungal programmed cell death | Contributor(s): | Katz, Margaret E (author); Braunberger, Katharyn S (author); Kelly, Joan M (author) | Publication Date: | 2016 | DOI: | 10.1016/j.fgb.2016.11.002 | Handle Link: | https://hdl.handle.net/1959.11/20078 | Abstract: | Apoptosis is a form of programmed cell death (PCD) that occurs during animal development and is also triggered by a variety of signals including nutrient or oxidative stress, hypoxia, DNA damage, viral infection and oncogenic transformation. Though apoptotic-like PCD also occurs in plants and fungi, genes encoding several of the key players in mammalian apoptosis (p53 and BH-domain proteins) have not been identified in these kingdoms. In this report we investigated whether HxkC, a mitochondrial hexokinase-like protein, and XprG, a putative p53-like transcription factor similar to Ndt80, play a role in programmed cell death in the filamentous fungus 'Aspergillus nidulans'. We show that a mutant lacking HxkC is more sensitive to oxidative stress. Autolysis, a form of fungal programmed cell death triggered by carbon starvation, is accelerated in the hxkC∆1 mutant but not the hxkC∆1 xprG∆1 double mutant. In the absence of nutrient stress, the hxkC∆1 mutant displays XprG-dependent DNA fragmentation typical of apoptosis and elevated levels of intracellular protease. HxkC and XprG are required for catabolism of N-acetylglucosamine, as in 'Trichoderma reesei'. We show that XprG is present in the nucleus. We conclude that, like mammalian mitochondrial hexokinase, HxkC has anti-apoptotic activity and the XprG transcription factor has a pro-apoptotic role in filamentous fungi. | Publication Type: | Journal Article | Source of Publication: | Fungal Genetics and Biology, v.97, p. 36-45 | Publisher: | Academic Press | Place of Publication: | United States of America | ISSN: | 1096-0937 1087-1845 |
Fields of Research (FoR) 2008: | 060503 Microbial Genetics 060405 Gene Expression (incl. Microarray and other genome-wide approaches) 060505 Mycology |
Fields of Research (FoR) 2020: | 310704 Microbial genetics 310505 Gene expression (incl. microarray and other genome-wide approaches) 310705 Mycology |
Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences | Socio-Economic Objective (SEO) 2020: | 280102 Expanding knowledge in the biological sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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