Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/13935
Title: | Green fluorescent protein expression triggers proteome changes in breast cancer cells | Contributor(s): | Coumans-Moens, Joelle (author) ; Gau, David (author); Poljak, Anne (author); Wassinger, Valerie (author); Roy, Partha (author); Moens, Pierre (author) | Publication Date: | 2014 | Open Access: | Yes | DOI: | 10.1016/j.yexcr.2013.07.019 | Handle Link: | https://hdl.handle.net/1959.11/13935 | Open Access Link: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866891 | Abstract: | Green fluorescent protein (GFP) is the most commonly used reporter of expression in cell biology despite evidence that it affects the cell physiology. The molecular mechanism of GFP-associated modifications has been largely unexplored. In this paper we investigated the proteome modifications following stable expression of GFP in breast cancer cells (MDA-MB-231). A combination of three different proteome analysis methods (2-DE, iTRAQ, label-free) was used to maximise proteome coverage. We found that GFP expression induces changes in expression of proteins that are associated with protein folding, cytoskeletal organisation and cellular immune response. In view of these findings, the use of GFP as a cell reporter should be carefully monitored. | Publication Type: | Journal Article | Source of Publication: | Experimental Cell Research, 320(1), p. 33-45 | Publisher: | Academic Press | Place of Publication: | United States of America | ISSN: | 1090-2422 0014-4827 |
Fields of Research (FoR) 2008: | 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) 111201 Cancer Cell Biology |
Fields of Research (FoR) 2020: | 310109 Proteomics and intermolecular interactions (excl. medical proteomics) 321101 Cancer cell biology |
Socio-Economic Objective (SEO) 2008: | 920102 Cancer and Related Disorders 970106 Expanding Knowledge in the Biological Sciences |
Socio-Economic Objective (SEO) 2020: | 280102 Expanding knowledge in the biological sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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