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Title: Green fluorescent protein expression triggers proteome changes in breast cancer cells
Contributor(s): Coumans-Moens, Joelle  (author)orcid ; Gau, David (author); Poljak, Anne (author); Wassinger, Valerie (author); Roy, Partha (author); Moens, Pierre  (author)orcid 
Publication Date: 2014
Open Access: Yes
DOI: 10.1016/j.yexcr.2013.07.019Open Access Link
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Abstract: Green fluorescent protein (GFP) is the most commonly used reporter of expression in cell biology despite evidence that it affects the cell physiology. The molecular mechanism of GFP-associated modifications has been largely unexplored. In this paper we investigated the proteome modifications following stable expression of GFP in breast cancer cells (MDA-MB-231). A combination of three different proteome analysis methods (2-DE, iTRAQ, label-free) was used to maximise proteome coverage. We found that GFP expression induces changes in expression of proteins that are associated with protein folding, cytoskeletal organisation and cellular immune response. In view of these findings, the use of GFP as a cell reporter should be carefully monitored.
Publication Type: Journal Article
Source of Publication: Experimental Cell Research, 320(1), p. 33-45
Publisher: Academic Press
Place of Publication: United States of America
ISSN: 0014-4827
Field of Research (FOR): 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)
111201 Cancer Cell Biology
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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