Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/13935
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dc.contributor.authorCoumans-Moens, Joelleen
dc.contributor.authorGau, Daviden
dc.contributor.authorPoljak, Anneen
dc.contributor.authorWassinger, Valerieen
dc.contributor.authorRoy, Parthaen
dc.contributor.authorMoens, Pierreen
dc.date.accessioned2014-02-06T14:01:00Z-
dc.date.issued2014-
dc.identifier.citationExperimental Cell Research, 320(1), p. 33-45en
dc.identifier.issn1090-2422en
dc.identifier.issn0014-4827en
dc.identifier.urihttps://hdl.handle.net/1959.11/13935-
dc.description.abstractGreen fluorescent protein (GFP) is the most commonly used reporter of expression in cell biology despite evidence that it affects the cell physiology. The molecular mechanism of GFP-associated modifications has been largely unexplored. In this paper we investigated the proteome modifications following stable expression of GFP in breast cancer cells (MDA-MB-231). A combination of three different proteome analysis methods (2-DE, iTRAQ, label-free) was used to maximise proteome coverage. We found that GFP expression induces changes in expression of proteins that are associated with protein folding, cytoskeletal organisation and cellular immune response. In view of these findings, the use of GFP as a cell reporter should be carefully monitored.en
dc.languageenen
dc.publisherAcademic Pressen
dc.relation.ispartofExperimental Cell Researchen
dc.titleGreen fluorescent protein expression triggers proteome changes in breast cancer cellsen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.yexcr.2013.07.019en
dcterms.accessRightsGreenen
dc.subject.keywordsProteomics and Intermolecular Interactions (excl Medical Proteomics)en
dc.subject.keywordsCancer Cell Biologyen
local.contributor.firstnameJoelleen
local.contributor.firstnameDaviden
local.contributor.firstnameAnneen
local.contributor.firstnameValerieen
local.contributor.firstnameParthaen
local.contributor.firstnamePierreen
local.subject.for2008060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)en
local.subject.for2008111201 Cancer Cell Biologyen
local.subject.seo2008920102 Cancer and Related Disordersen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Rural Medicineen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailjmoensco@une.edu.auen
local.profile.emailpmoens@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20130822-10013en
local.publisher.placeUnited States of Americaen
local.format.startpage33en
local.format.endpage45en
local.identifier.scopusid84888205565en
local.url.openhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3866891en
local.peerreviewedYesen
local.identifier.volume320en
local.identifier.issue1en
local.access.fulltextYesen
local.contributor.lastnameCoumans-Moensen
local.contributor.lastnameGauen
local.contributor.lastnamePoljaken
local.contributor.lastnameWassingeren
local.contributor.lastnameRoyen
local.contributor.lastnameMoensen
dc.identifier.staffune-id:jmoenscoen
dc.identifier.staffune-id:pmoensen
local.profile.orcid0000-0001-6642-5202en
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:14148en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleGreen fluorescent protein expression triggers proteome changes in breast cancer cellsen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorCoumans-Moens, Joelleen
local.search.authorGau, Daviden
local.search.authorPoljak, Anneen
local.search.authorWassinger, Valerieen
local.search.authorRoy, Parthaen
local.search.authorMoens, Pierreen
local.uneassociationUnknownen
local.identifier.wosid000327911900004en
local.year.published2014en
local.subject.for2020310109 Proteomics and intermolecular interactions (excl. medical proteomics)en
local.subject.for2020321101 Cancer cell biologyen
local.subject.seo2020280102 Expanding knowledge in the biological sciencesen
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