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https://hdl.handle.net/1959.11/13494
Title: | Analysis of the pobA and pobR genes controlling expression of p-hydroxybenzoate hydroxylase in 'Azotobacter chroococcum' | Contributor(s): | Quinn, Jennifer A (author); McKay, David B (author); Entsch, Barrie (author) | Publication Date: | 2001 | DOI: | 10.1016/S0378-1119(00)00599-0 | Handle Link: | https://hdl.handle.net/1959.11/13494 | Abstract: | We report the cloning and analysis of a gene and its cognate regulatory element from a member of the 'Azotobacteriaceae' which are involved in the breakdown of an aromatic compound. The genes from 'Azotobacter chroococcum' encoding p-hydroxybenzoate hydroxylase (pobA) and its regulatory protein (pobR) were cloned from a genomic library and sequenced. Sequence analysis of pobA revealed homology with other bacterial p-hydroxybenzoate hydroxylase enzymes. Residues essential to the structure and function of the enzyme have been conserved. The pobR gene encodes a DNA binding regulatory protein with similarity to proteins from the AraC/XylS family of transcriptional activators. A fragment containing both pobA and pobR was cloned into pUC19 and p-hydroxybenzoate hydroxylase activity was induced in 'Escherichia coli' by the addition of p-hydroxybenzoate. A frame-shift mutation introduced into the pobR gene prevented expression of p-hydroxybenzoate hydroxylase, indicating that PobR is the protein required for transcription of pobA. Interestingly, 'A. chroococcum' PobR has no homology to the PobR protein that is the transcriptional activator of pobA in 'Acinetobacter' strain ADP1, a protein that is homologous to the IclR family of transcriptional regulators. However, PobR from 'A. chroococcum' is homologous to several other proteins, suggesting that these proteins will also function as transcriptional activators of pobA. | Publication Type: | Journal Article | Grant Details: | ARC/A09906323 | Source of Publication: | Gene, 264(1), p. 77-85 | Publisher: | Elsevier BV | Place of Publication: | Netherlands | ISSN: | 1879-0038 0378-1119 |
Fields of Research (FoR) 2008: | 060503 Microbial Genetics 060405 Gene Expression (incl Microarray and other genome-wide approaches) 060107 Enzymes |
Socio-Economic Objective (SEO) 2008: | 970106 Expanding Knowledge in the Biological Sciences | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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