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DatasetPublication Modelling conflict_Hinds_Dissertation2024_Appendix D, E(University of New England, 2025)These datasets were created as part of Rhonda Hinds’ 2024 HASS 218 project investigating the palimpsest as a framework for interrogating modern conflict landscapes. This was a desktop investigation, utilising text, photographic and cartographic material as information from which the datasets were created. The PDF document reflects the appearance of the data as Appendices D and E in Hinds’ 2024 dissertation. - Some of the metrics are blocked by yourconsent settings
Awaiting ReviewPublication Role of SERPINI1 in the retinal function and determining the effects of oxidative stress on serpin expression(2019-07)Purpose : SERPINI1 is a member of serine protease inhibitor family and is expressed in neuronal tissues such as brain and retina. SERPINI1 upregulation in retina has been shown to be neuroprotective in acute ischemic injury. This study investigated the effects of SERPINI1 neutralisation on inner retinal function in healthy animal eyes. SERPINI1 is susceptible to oxidative stress, therefore we also investigated the effects of oxidative stress on SERPINI1 expression changes in 661W photoreceptor cells and change in autophagy markers.Methods : SERPINI1 or IgG antibody was administered intravitreally in healthy mice retina (weekly once for 8 weeks, 2µl, n=20). ERGs and positive scotopic threshold response (pSTR) was measured using Phoenix Ganzfield (-4.3log cd s/m2) after 8 weeks. The amplitude of each component was measured and analysed. 661W photoreceptor cells were cultured, incubated with hydrogen-peroxide (10µM) and harvested after 8 and 24 hours. Western blotting was evaluated SERPINI1 and expression of autophagy markers.Results : The SERPINI1 neutralisation by intravitreal injection of anti-SERPINI1 in C57BL/6 mice retinas showed no change in ERG but significant loss of pSTR amplitude as compared to IgG injected or no-injection control counterparts: mean±SEM; control vs IgG vs anti-SERPINI; 51.2±3.42 µV vs 47.02±6.03 µV vs 30.37±4.12 µV (p<0.007). Western blot showed that SERPINI1 was expressed in 661W cells and was significantly elevated after 8 hrs (81.81±9.43 vs 227.7±0.72; p<0.0001, n=3) but its expression reduced at 24 hrs following H2O2 treatment. H2O2 also significantly enhanced Beclin1 (p<0.0008), LC3II (p<0.04), Atg3 (p<0.05), Atg7 (p<0.02) and Atg16 L1 (p<0.03) after 8 hrs and expression of some of these markers was then reduced after 24 hrs. Therefore, there was an early increase in SERPINI1 in response to oxidative stress which paralleled the change in autophagy markers.Conclusions : Blocking SERPINI1 in healthy retinal neurons diminished inner retinal function as determined by pSTR amplitude reduction. The mechanism for this is unknown but may reflect increased excitotoxicity or synaptic dysfunction. Further studies are needed to clarify relationship between SERPINI1 and autophagy in photoreceptor cells. Long term oxidative stress may induce changes in SERPINI1 levels that could render neurons more susceptible to degenerative change. - Some of the metrics are blocked by yourconsent settings
Journal ArticlePublication One protein, multiple pathologies: multifaceted involvement of amyloid β in neurodegenerative disorders of the brain and retina(Springer Cham, 2016-11)Accumulation of amyloid β (Aβ) and its aggregates in the ageing central nervous system is regarded synonymous to Alzheimer’s disease (AD) pathology. Despite unquestionable advances in mechanistic and diagnostic aspects of the disease understanding, the primary cause of Aβ accumulation as well as its in vivo roles remains elusive; nonetheless, the majority of the efforts to address pathological mechanisms for therapeutic development are focused towards moderating Aβ accumulation in the brain. More recently, Aβ deposition has been identified in the eye and is linked with distinct age-related diseases including age-related macular degeneration, glaucoma as well as AD. Awareness of the Aβ accumulation in these markedly different degenerative disorders has led to an increasing body of work exploring overlapping mechanisms, a prospective biomarker role for Aβ and the potential to use retina as a model for brain related neurodegenerative disorders. Here, we present an integrated view of current understanding of the retinal Aβ deposition discussing the accumulation mechanisms, anticipated impacts and outlining ameliorative approaches that can be extrapolated to the retina for potential therapeutic benefits. Further longitudinal investigations in humans and animal models will determine retinal Aβ association as a potential pathognomonic, diagnostic or prognostic biomarker.
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Journal ArticlePublication PTPN11 induces endoplasmic stress and apoptosis in SH-SY5Y cells(Elsevier Ltd, 2017-11-19)PTPN11 is associated with regulation of growth factor signaling pathways in neuronal cells. Using SH-SY5Y neuroblastoma cells, we showed that adeno-associated virus (AAV)-mediated PTPN11 upregulation was associated with TrkB antagonism, reduced neuritogenesis and enhanced endoplasmic reticulum (ER) stress response leading to apoptotic changes. Genetic knock-down of PTPN11 on the other hand leads to increased TrkB phosphorylation in SH-SY5Y cells. ER stress response induced by PTPN11 upregulation was alleviated pharmacologically by a TrkB agonist. Conversely the enhanced ER stress response induced by TrkB receptor antagonism was ameliorated by PTPN11 suppression, providing evidence of cross-talk of PTPN11 effects with TrkB actions. BDNF treatment of neuronal cells with PTPN11 upregulation also resulted in reduced expression of ER stress protein markers. This study provides evidence of molecular interactions between PTPN11 and the TrkB receptor in SH-SY5Y cells. The results reinforce the role played by PTPN11 in regulating neurotrophin protective signaling in neuronal cells and highlight that PTPN11 dysregulation promotes apoptotic activation. Based on these findings we suggest that blocking PTPN11 could have potential beneficial effects to limit the progression of neuronal loss in neurodegenerative disorders.
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Publication Open AccessJournal ArticleGlaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin(Nature Publishing Group, 2017-08-21)Neuroserpin is a serine protease inhibitor that regulates the activity of plasmin and its activators in the neuronal tissues. This study provides novel evidence of regulatory effect of the neuroserpin on plasmin proteolytic activity in the retina in glaucoma. Human retinal and vitreous tissues from control and glaucoma subjects as well as retinas from experimental glaucoma rats were analysed to establish changes in plasmin and neuroserpin activity. Neuroserpin undergoes oxidative inactivation in glaucoma which leads to augmentation of plasmin activity. Neuroserpin contains several methionine residues in addition to a conserved reactive site methionine and our study revealed enhanced oxidation of Met residues in the serpin under glaucoma conditions. Met oxidation was associated with loss of neuroserpin inhibitory activity and similar findings were observed in the retinas of superoxide dismutase (SOD) mutant mice that have increased oxidative stress. Treatment of purified neuroserpin with H2O2 further established that Met oxidation inversely correlated with its plasmin inhibitory activity. Dysregulation of the plasmin proteolytic system associated with increased degradation of the extracellular matrix (ECM) proteins in the retina. Collectively, these findings delineate a novel molecular basis of plasmin activation in glaucoma and potentially for other neuronal disorders with implications in disease associated ECM remodelling.
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Conference PublicationPublication Orgasmic Gushing: where does the fluid come from and how is it produced?(Women in Research (WiR), 2005)There are three sexual fluids from women: lubrication (e.g. transudation of fluid across the mucosa of the vagina, and mucus from the greater vestibular glands); female ejaculation (from paraurethral glands); and gushing. Orthodox western medicine and physiology does not yet have a standardized description or explanation for the third, gushing. The present paper proposes that the gushing fluid is a filtrate of plasma, produced by the mechanism known as transudation. This is an additional application for the transudation mechanism, after the well accepted roles in lubrication of the vagina, and in generating serous fluids. The present model proposes that the fluid released in a gush arises from the ventral wall of the vagina due to the presence there of increased surface area of mucosa, dilated arterioles, pressurized venous and lymphatic plexuses, and compression provided by muscle contraction during orgasm.64971 - Some of the metrics are blocked by yourconsent settings
DatasetPublication Mapping Long Term Changes in Mangrove Cover and Predictions of Future Change under Different Climate Change Scenarios in the Sundarbans, Bangladesh(2018-05-22)Ground-based readings of temperature and rainfall, satellite imagery, aerial photographs, ground verification data and Digital Elevation Model (DEM) were used in this study. Ground-based meteorological information was obtained from Bangladesh Meteorological Department (BMD) for the period 1977 to 2015 and was used to determine the trends of rainfall and temperature in this thesis. Satellite images obtained from the US Geological Survey (USGS) Center for Earth Resources Observation and Science (EROS) website (www.glovis.usgs.gov) in four time periods were analysed to assess the dynamics of mangrove population at species level. Remote sensing techniques, as a solution to lack of spatial data at a relevant scale and difficulty in accessing the mangroves for field survey and also as an alternative to the traditional methods were used in monitoring of the changes in mangrove species composition, . To identify mangrove forests, a number of satellite sensors have been used, including Landsat TM/ETM/OLI, SPOT, CBERS, SIR, ASTER, and IKONOS and Quick Bird. The use of conventional medium-resolution remote sensor data (e.g., Landsat TM, ASTER, SPOT) in the identification of different mangrove species remains a challenging task. In many developing countries, the high cost of acquiring high- resolution satellite imagery excludes its routine use. The free availability of archived images enables the development of useful techniques in its use and therefor Landsat imagery were used in this study for mangrove species classification. Satellite imagery used in this study includes: Landsat Multispectral Scanner (MSS) of 57 m resolution acquired on 1st February 1977, Landsat Thematic Mapper (TM) of 28.5 m resolution acquired on 5th February 1989, Landsat Enhanced Thematic Mapper (ETM+) of 28.5 m resolution acquired on 28th February 2000 and Landsat Operational Land Imager (OLI) of 30 m resolution acquired on 4th February 2015. To study tidal channel dynamics of the study area, aerial photographs from 1974 and 2011, and a satellite image from 2017 were used. Satellite images from 1974 with good spatial resolution of the area were not available, and therefore aerial photographs of comparatively high and fine resolution were considered adequate to obtain information on tidal channel dynamics. Although high-resolution satellite imagery was available for 2011, aerial photographs were used for this study due to their effectiveness in terms of cost and also ease of comparison with the 1974 photographs. The aerial photographs were sourced from the Survey of Bangladesh (SOB). The Sentinel-2 satellite image from 2017 was downloaded from the European Space Agency (ESA) website (https://scihub.copernicus.eu/). In this research, elevation data acts as the main parameter in the determination of the sea level rise (SLR) impacts on the spatial distribution of the future mangrove species of the Bangladesh Sundarbans. High resolution elevation data is essential for this kind of research where every centimeter counts due to the low-lying characteristics of the study area. The high resolution (less than 1m vertical error) DEM data used in this study was obtained from Water Resources Planning Organization (WRPO), Bangladesh. The elevation information used to construct the DEM was originally collected by a Finnish consulting firm known as FINNMAP in 1991 for the Bangladesh government.48193 50 - Some of the metrics are blocked by yourconsent settings
Conference PublicationPublication Reinforced Behavioral Variability and Sequence Learning Across Species(Association for Behavior Analysis International (ABAI), 2012)Previous research shows that reinforcement of variable responding will facilitate sequence learning in rats (Neuringer, Deiss & Olson, 2000) but may interfere with sequence learning in humans (Maes & van der Goot, 2006). The present study aimed to replicate and extend previous research by assessing the role of behavioral variability in the learning of difficult target sequences across 3 species: humans (n = 60), hens (n = 18) and possums (n = 6). Participants were randomly allocated to one of three experimental conditions (Control, Variable, Any). In the Control conditions sequences were only reinforced if they were the target sequence, in the Variability conditions sequences were concurrently reinforced on a Variable Interval 60-s schedule if the just entered sequence met a variability criterion, and in the Any condition sequences were concurrently reinforced on a Variable Interval 60-s schedule for any sequence entered. The results support previous findings with animals and humans; hens and possums were more likely to learn the target sequence in the Variability condition, and human participants were more likely to learn the target sequence in the Control condition. Possible explanations for differences between the performance of humans and animals on this task will be discussed.39889 1 - Some of the metrics are blocked by yourconsent settings
Publication Open AccessDatasetThe drivers and consequences of change to the physical character of waterholes on an Australian dryland river(University of New England, 2021-08-27)This dataset provides all the raw and analysed data for the thesis titled 'The drivers and consequences of change to the physical character of waterholes on an Australian dryland river'.
The data has been divided into four folders that are aligned with the data chapters for the thesis. These being: (Ch 2) waterhole mapping, (Ch 3) floodplain gullies, (Ch 4) sediment transport and (Ch 5) fish.
A README file is provided for each chapter which contains a description of the individual datasets and a list of files that make up each dataset.
The data in this archive is a combination of data obtained from desktop studies as well as field work on the Darling River (i.e., the fish data).
Further, fish data were collected on the Darling River between Bourke and Wilcannia. Waterhole mapping was undertaken on the Barwon-Darling between Walgett and Wilcannia. Gully mapping was undertaken on the Barwon-Darling River between Mungindi and Wilcannia. Sediment transport capacity was assessed at five sites between Collarenebri and Tilpa.37752 2877 - Some of the metrics are blocked by yourconsent settings
Publication Open AccessJournal ArticleA Review into Effective Classroom Management and Strategies for Student Engagement: Teacher and Student Roles in Today’s Classrooms(Redfame Publishing Inc, 2019-12)A teacher's role encompasses far more than just imparting curricula outcomes to their students: they need to equip students with the necessary tools to experience social and academic success both inside the classroom and beyond it. Teachers need to empower students with the means to critically analyse the world around them in order to develop into critical independent thinkers. Students need to be proficient in utilising skills associated with higher levels of thinking, that will empower them with the ability to identify, analyse and evaluate the infinite volume of information available through our rapidly changing digital world. Just as teachers need to take responsibility for the various methods of teaching and instruction in the classroom, it is essential for students to take ownership of the learning process, to ensure future success in university environments, where sustained personal effort and metacognitive skills are fundamental to academic success. The object of the review of the literature surrounding the roles of teacher and student, effective classroom management strategies, and successful evidence-based teaching and learning pedagogies, is to assist new and experienced teachers in the promotion of a positive classroom experience for all.29849 47695
