Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/61604
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lu, Jamie F | en |
dc.contributor.author | Pokharel, Deep | en |
dc.contributor.author | Padula, Matthew P | en |
dc.contributor.author | Bebawy, Mary | en |
dc.date.accessioned | 2024-07-12T08:13:05Z | - |
dc.date.available | 2024-07-12T08:13:05Z | - |
dc.date.issued | 2016-11 | - |
dc.identifier.citation | Journal of biochemistry, 160(5), p. 281-289 | en |
dc.identifier.issn | 1756-2651 | en |
dc.identifier.issn | 0021-924X | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/61604 | - |
dc.description.abstract | <p>Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology available in assessing protein translation following homotypic intercellular transfer of nucleic acids. Using surface peptide shaving we have demonstrated that MVs derived from human leukaemic cells transfer functional P-glycoprotein transcripts, conferring drug-efflux capacity to recipient cells. We demonstrate expression of newly synthesized protein using Western blot. Furthermore, we show functionality of translated P-gp protein in recipient cells using Calcein-AM dye exclusion assays on flow cytometry. Newly synthesized 170 kDa P-gp was detected in recipient cells after coculture with shaven MVs and these proteins were functional, conferring drug efflux. This is the first demonstration of functionality of transferred nucleic acids between human homotypic cells as well as the translation of the cancer multidrug-resistance protein in recipient cells following intercellular transfer of its transcript. This study supports the significant role of MV’s in the transfer of deleterious traits in cancer populations and describes a new paradigm in mechanisms governing the acquisition of traits in cancer cell populations.</p> | en |
dc.language | en | en |
dc.publisher | Oxford University Press | en |
dc.relation.ispartof | Journal of biochemistry | en |
dc.title | A novel method to detect translation of membrane proteins following microvesicle intercellular transfer of nucleic acids | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1093/jb/mvw033 | en |
local.contributor.firstname | Jamie F | en |
local.contributor.firstname | Deep | en |
local.contributor.firstname | Matthew P | en |
local.contributor.firstname | Mary | en |
local.relation.isfundedby | NHMRC | en |
local.profile.school | School of Psychology | en |
local.profile.email | mbebawy@une.edu.au | en |
local.output.category | C1 | en |
local.grant.number | APP1007613 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | United Kingdom | en |
local.format.startpage | 281 | en |
local.format.endpage | 289 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 160 | en |
local.identifier.issue | 5 | en |
local.contributor.lastname | Lu | en |
local.contributor.lastname | Pokharel | en |
local.contributor.lastname | Padula | en |
local.contributor.lastname | Bebawy | en |
dc.identifier.staff | une-id:mbebawy | en |
local.profile.orcid | 0000-0003-2606-921X | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:1959.11/61604 | en |
local.date.onlineversion | 2016-05-06 | - |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | A novel method to detect translation of membrane proteins following microvesicle intercellular transfer of nucleic acids | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.relation.grantdescription | NHMRC/APP1007613 | en |
local.search.author | Lu, Jamie F | en |
local.search.author | Pokharel, Deep | en |
local.search.author | Padula, Matthew P | en |
local.search.author | Bebawy, Mary | en |
local.uneassociation | No | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.year.available | 2016 | en |
local.year.published | 2016 | en |
local.fileurl.closedpublished | https://rune.une.edu.au/web/retrieve/76e7e5ac-8af7-4c28-9f89-35679aed075c | en |
local.subject.for2020 | 3208 Medical physiology | en |
local.profile.affiliationtype | External Affiliation | en |
local.profile.affiliationtype | External Affiliation | en |
local.profile.affiliationtype | External Affiliation | en |
local.profile.affiliationtype | External Affiliation | en |
local.date.moved | 2024-08-01 | en |
Appears in Collections: | Journal Article School of Psychology |
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