Supplementation of live yeast culture modulates intestinal health, immune responses, and microbiota diversity in broiler chickens

Abstract

<p>The present study investigated the effects of live yeast cultures (LYC) on growth performance, gut health indicators, and immune responses in broiler chickens. A total of 720 mixed-sex broilers (40 birds/pen; 9 replicates/treatment) were randomly allocated to two dietary treatments: (1) a basal diet based on corn–soybean meal (CON) and (2) CON with 1 g/kg LYC. At 35 d of age, one bird per replicate pen was chosen for biopsy. LYC group tended (<i>P</i> < 0.10) to increase average daily gain during the grower phase compared with CON group. Broilers fed LYC diet had increased (<i>P</i> = 0.046) duodenal villus height and area but reduced (<i>P</i> = 0.003) duodenal crypt depth compared with those fed CON diet. Birds fed LYC diet presented alleviated (<i>P</i> < 0.05) serum TNF-α, IL-1β, and IL-6 levels compared with those fed CON diet. Further, birds fed LYC diet exhibited upregulated (<i>P</i> < 0.05) ileal tight junction-related proteins and pro-inflammatory cytokines in the ileal tissue compared with those fed CON diet. Inverse Simpson’s diversity (<i>P</i> = 0.038) revealed that birds fed CON diet had a more diverse microbiota community in the ileal digesta, compared with those fed LYC diet, while no significant difference between the treatments on Chao1 and Shannon’s indices was observed. Based on the weighted UniFrac distance, the PCoA showed that microbiota in the ileal digesta of the LYC group was different from that of the CON group. LYC group increased the abundance of the phyla Firmicutes and genera <i>Lactobacillus, Prevotella</i>, and <i>Enterococcus</i> compared with CON group. The present study demonstrated that supplemental LYC as a feed additive provide supportive effects on enhancing gut functionality by improving the upper intestinal morphology and gut integrity, and modulating the immune system and microbiota communities of birds.</p>