Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/60441
Title: Seasonal distribution and phylogenetic analysis of human metapneumovirus in children with acute respiratory tract infections admitted to a general hospital in Sri Lanka
Contributor(s): Divarathna, M V M (author); Rafeek, R A M  (author)orcid ; Morel, A J (author); Jagoda, S (author); Noordeen, F (author)
Publication Date: 2021-10
Open Access: Yes
DOI: 10.4038/sljid.v11i0.8453Open Access Link
Handle Link: https://hdl.handle.net/1959.11/60441
Abstract: 

Introduction and Objectives:Human metapneumovirus (hMPV) is one of the major causes of acute respiratory tract infections (ARTI) in children worldwide. hMPV has two major types, A and B and four subtypes, A1, A2, B1, and B2.hMPV A2 is further divided into three sub-genotypes, A2a, A2b and A2c. This study was undertaken to determine the distribution, seasonality and subtypes of hMPV circulating in children <5 years with ARTI.

Methods:A total of 500 nasopharyngeal aspirates were collected from children <5 years hospitalized at the District General Hospital, Kegalle between May 2016 and July 2018. hMPV was detected by a conventional reverse transcription PCR (RT-PCR). Of the 119 hMPV positive children’s PCR products, 17 randomly selected samples were characterized using direct Sanger sequencing (n=15) and Thymine and Adenine (TA) cloning followed by Sanger sequencing (n=2). The sample sequencing was limited to 17 due to funding restraints.

Results: hMPV was detected with a prevalence of 23.8% (119/500) in children < 5 years of age throughout the study period with two major peaks occurring in February to May in 2017 and May to July in 2018 and two minor peaks occurring in July 2017 and February 2018. The sequenced hMPV strains belonged to type A and the subtype A2. It is not clear whether the A2 strains belong to the sub-genotypes A2b or A2c.

Conclusions:hMPV infection had a prevalence of ~24% and is considered high compared to its prevalence in some other countries. The infection was distributed throughout the year in the study area. The sequenced 17 hMPV samples were hMPV A2 genotype.

Publication Type: Conference Publication
Conference Details: CSS 2021: 10th Annual conference and scientific sessions, virtual event, 22nd October, 2021
Source of Publication: Sri Lankan Journal of Infectious Diseases, v.11
Publisher: University of Colombo, Faculty of Medicine
Place of Publication: Sri Lanka
ISSN: 2448-9654
2012-8169
Fields of Research (FoR) 2020: 3201 Cardiovascular medicine and haematology
Peer Reviewed: Yes
HERDC Category Description: E3 Extract of Scholarly Conference Publication
Appears in Collections:Conference Publication
School of Science and Technology

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