Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/51541
Title: Antimicrobial peptide, cLF36, affects performance and intestinal morphology, microflora, junctional proteins, and immune cells in broilers challenged with E. coli
Contributor(s): Daneshmand, Ali  (author); Kermanshahi, Hassan (author); Sekhavati, Mohammad Hadi (author); Javadmanesh, Ali (author); Ahmadian, Monireh (author)
Publication Date: 2019-10-02
Open Access: Yes
DOI: 10.1038/s41598-019-50511-7
Handle Link: https://hdl.handle.net/1959.11/51541
Abstract: This study investigated the effects of an antimicrobial peptide (AMP), cLF36, on growth performance and the histophysiological changes of the intestine in E. coli-challenged broiler chickens. A total number of 360 day old male chicks were randomly assigned to 4 groups of 6 replicates as follows: T1) negative control diet based on corn-soybean meal without E. coli challenge and additives; T2) positive control diet based on corn-soybean meal and challenged with E. coli without any additives; T3) positive control diet challenged with E. coli and supplemented with 20mg AMP (cLF36)/kg diet; T4) positive control diet challenged with E. coli and supplemented with 45mg antibiotic (bacitracin methylene disalicylate)/kg diet. Results showed that T3 improved growth performance and the jejunal morphology of E. coli-challenged chickens similar to those of T4. While antibiotic non-selectively decreased the population of ileal bacteria, AMP increased the population of Lactobacillus spp. and decreased harmful bacteria in the ileum of E. coli-challenged chickens. Supplementing E. coli-challenged chickens with AMP improved the gene expression of immune cells and upregulated the expression of tight junction proteins compared to other challenged groups. In conclusion, although cLF36 beneficially affected growth performance and the intestinal morphology of E. coli-challenged chickens similar to those of the antibiotic group, this AMP drastically improved the intestinal microbiome, immune cells, and junctional proteins compared to other E. coli-challenged birds, and can be nominated as an alternative for growth promoter antibiotics.
Publication Type: Journal Article
Source of Publication: Scientific Reports, v.9, p. 1-9
Publisher: Nature Publishing Group
Place of Publication: United Kingdom
ISSN: 2045-2322
Fields of Research (FoR) 2020: 300303 Animal nutrition
Socio-Economic Objective (SEO) 2020: 280101 Expanding knowledge in the agricultural, food and veterinary sciences
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science

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