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Title: Detection and quantification of Marek's disease viruses using real-time polymerase chain reaction in separate and duplex assays
Contributor(s): Islam, Aminul (author); Harrison, B (author); Cheetham, Brian Frederick  (author); Mahony, T J (author); Young, P F (author); Walkden-Brown, Steve William  (author)orcid 
Publication Date: 2004
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Abstract: Development and initial validation of quantitative real-time PCR (qPCR) assays for the three serotypes of Marek's Disease Virus (MDV) are described. Also described is the development of an internal control qPCR assay that detects the chicken α2(VI) collagen gene. To reduce costs, a duplex assay for MDV1 and the internal control was also developed. The MDV qPCR assays were specific to their target gene and more sensitive than standard PCR when compared using Australian field and vaccine strains of MDV. All assays were found to have acceptable reproducibility. Relative abundance (RA) of MDVI and HVT viruses was quantified using the relative standard curve method in twenty experimentally infected chickens over a period of 7-35 days post infection and was shown to vary during the course of the infection. These qPCR assays will be useful for reliable differentiation and quantitation of MDV for a variety of applications.
Publication Type: Conference Publication
Conference Details: Australian Poultry Science Symposium, Sydney, NSW, Australia, 9 - 11 February, 2004
Source of Publication: Proceedings of Australian Poultry Science Symposium, v.16, p. 153-156
Publisher: Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch
Place of Publication: Sydney, NSW, Australia
ISSN: 1034-6260
Field of Research (FOR): 060506 Virology
Socio-Economic Objective (SEO): 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
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