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https://hdl.handle.net/1959.11/42308
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bindari, Yugal R | en |
dc.contributor.author | Moore, Robert J | en |
dc.contributor.author | Van, Thi Thu Hao | en |
dc.contributor.author | Hilliar, Matthew | en |
dc.contributor.author | Wu, Shu-Biao | en |
dc.contributor.author | Walkden-Brown, Stephen W | en |
dc.contributor.author | Gerber, Priscilla F | en |
dc.date.accessioned | 2022-02-14T04:04:21Z | - |
dc.date.available | 2022-02-14T04:04:21Z | - |
dc.date.issued | 2021-08-05 | - |
dc.identifier.citation | PLoS One, 16(8), p. 1-17 | en |
dc.identifier.issn | 1932-6203 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/42308 | - |
dc.description.abstract | <p>Traditional sampling methods for the study of poultry gut microbiota preclude longitudinal studies as they require euthanasia of birds for the collection of caecal and ileal contents. Some recent research has investigated alternative sampling methods to overcome this issue. The main goal of this study was to assess to what extent the microbial composition of non-invasive samples (excreta, litter and poultry dust) are representative of invasive samples (caecal and ileal contents). The microbiota of excreta, dust, litter, caecal and ileal contents (n = 110) was assessed using 16S ribosomal RNA gene amplicon sequencing. Of the operational taxonomic units (OTUs) detected in caecal contents, 99.7% were also detected in dust, 98.6% in litter and 100% in excreta. Of the OTUs detected in ileal contents, 99.8% were detected in dust, 99.3% in litter and 95.3% in excreta. Although the majority of the OTUs found in invasive samples were detected in non-invasive samples, the relative abundance of members of the microbial communities of these groups were different, as shown by beta diversity measures. Under the conditions of this study, correlation analysis showed that dust could be used as a proxy for ileal and caecal contents to detect the abundance of the phylum <i>Firmicutes</i>, and excreta as a proxy of caecal contents for the detection of <i>Tenericutes</i>. Similarly, litter could be used as a proxy for caecal contents to detect the abundance of <i>Firmicutes</i> and <i>Tenericutes</i>. However, none of the non-invasive samples could be used to infer the overall abundance of OTUs observed in invasive samples. In conclusion, non-invasive samples could be used to detect the presence and absence of the majority of the OTUs found in invasive samples, but could not accurately reflect the microbial community structure of invasive samples.</p> | en |
dc.language | en | en |
dc.publisher | Public Library of Science | en |
dc.relation.ispartof | PLoS One | en |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Microbial communities of poultry house dust, excreta and litter are partially representative of microbiota of chicken caecum and ileum | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1371/journal.pone.0255633 | en |
dc.identifier.pmid | 34351989 | en |
dcterms.accessRights | UNE Green | en |
local.contributor.firstname | Yugal R | en |
local.contributor.firstname | Robert J | en |
local.contributor.firstname | Thi Thu Hao | en |
local.contributor.firstname | Matthew | en |
local.contributor.firstname | Shu-Biao | en |
local.contributor.firstname | Stephen W | en |
local.contributor.firstname | Priscilla F | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | ybindari@myune.edu.au | en |
local.profile.email | mhilliar@myune.edu.au | en |
local.profile.email | swu3@une.edu.au | en |
local.profile.email | swalkden@une.edu.au | en |
local.profile.email | pgerber2@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | United States of America | en |
local.identifier.runningnumber | e0255633 | en |
local.format.startpage | 1 | en |
local.format.endpage | 17 | en |
local.identifier.scopusid | 85112645963 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 16 | en |
local.identifier.issue | 8 | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Bindari | en |
local.contributor.lastname | Moore | en |
local.contributor.lastname | Van | en |
local.contributor.lastname | Hilliar | en |
local.contributor.lastname | Wu | en |
local.contributor.lastname | Walkden-Brown | en |
local.contributor.lastname | Gerber | en |
dc.identifier.staff | une-id:mhillia2 | en |
dc.identifier.staff | une-id:swu3 | en |
dc.identifier.staff | une-id:swalkden | en |
dc.identifier.staff | une-id:pgerber2 | en |
local.profile.orcid | 0000-0001-7292-0007 | en |
local.profile.orcid | 0000-0002-1790-6015 | en |
local.profile.orcid | 0000-0002-0638-5533 | en |
local.profile.orcid | 0000-0002-8343-8299 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:1959.11/42308 | en |
dc.identifier.academiclevel | Student | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Student | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Microbial communities of poultry house dust, excreta and litter are partially representative of microbiota of chicken caecum and ileum | en |
local.relation.fundingsourcenote | Poultry Hub Australia (grant 18-424) partially funded this research (PFG) | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Bindari, Yugal R | en |
local.search.author | Moore, Robert J | en |
local.search.author | Van, Thi Thu Hao | en |
local.search.author | Hilliar, Matthew | en |
local.search.author | Wu, Shu-Biao | en |
local.search.author | Walkden-Brown, Stephen W | en |
local.search.author | Gerber, Priscilla F | en |
local.open.fileurl | https://rune.une.edu.au/web/retrieve/5c27dcdd-683b-4610-abad-73123a8b7b13 | en |
local.uneassociation | Yes | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.identifier.wosid | 000685265700054 | en |
local.year.published | 2021 | en |
local.fileurl.open | https://rune.une.edu.au/web/retrieve/5c27dcdd-683b-4610-abad-73123a8b7b13 | en |
local.fileurl.openpublished | https://rune.une.edu.au/web/retrieve/5c27dcdd-683b-4610-abad-73123a8b7b13 | en |
local.subject.for2020 | 300304 Animal protection (incl. pests and pathogens) | en |
local.subject.for2020 | 300903 Veterinary bacteriology | en |
local.subject.for2020 | 300303 Animal nutrition | en |
local.subject.seo2020 | 100411 Poultry | en |
Appears in Collections: | Journal Article School of Environmental and Rural Science |
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File | Description | Size | Format | |
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openpublished/MicrobialBindariHilliarWuWalkdenBrownGerber2021JournalArticle.pdf | Published version | 968.89 kB | Adobe PDF Download Adobe | View/Open |
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