Spatial and temporal distribution of Culicoides species in the New England region of New South Wales, Australia between 1990 and 2018

Abstract

<p><i>Culicoides</i> are one of the smallest hematophagous flies measuring 1-5 mm in size with only females seeking blood for egg development. The present study investigated spatio-temporal distribution of <i>Culicoides</i> species trapped between 1990 and 2018 at 13 sites in the New England region of NSW, Australia using automated light traps. Trapping locations were divided into three subregions (tablelands, slopes and plains). Nineteen <i>Culicoides</i> species were identified. <i>Culicoides marksi</i> and <i>C. austropalpalis</i> were the most abundant and widespread species. <i>Culicoides brevitarsis</i>, the principal vector of livestock diseases in New South Wales comprised 2.9% of the total catch and was detected in 12 of the 13 locations in the study. Abundance as determined by Log₁₀ <i>Culicoides</i> count per trapping event for the eight most abundant species did not vary significantly with season but trended towards higher counts in summer for <i>C. marksi</i> (P = 0.09) and <i>C. austropalpalis</i> (P = 0.05). Significant geographic variation in abundance was observed for <i>C. marksi</i>, <i>C. austropalpalis</i> and <i>C. dycei</i> with counts decreasing with increasing altitude from the plains to the slopes and tablelands. <i>Culicoides victoriae</i> exhibited the reverse trend in abundance (P = 0.08). Greater abundance during the warmer seasons and at lower altitudes for <i>C. marksi</i> and <i>C. austropalpalis</i> was indicative of temperature and rainfall dependence in this region with moderate summer dominance in rainfall. The Shannon-Wiener diversity index of species was higher on the tablelands (H = 1.59) than the slopes (H = 1.33) and plains (H = 1.08) with evenness indices of 0.62, 0.46 and 0.39 respectively. <i>Culicoides</i> species on the tablelands were more diverse than on the slopes and plains where <i>C. marksi</i> and <i>C. austropalpalis</i> dominated. The temporal and spatial variation in abundance, diversity and evenness of species reported in this diverse region of Australia provides additional insight into <i>Culicoides</i> as pests and disease vectors and may contribute to future modelling studies.</p>