Rapid Communication: mapping of the Ca²⁺ 'ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum' (ATP2A1) gene to porcine chromosome 3

Abstract

Locus Name: Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum. Species: Sus scrofa. Locus Symbol: AMAL Source of Primers: Two sets of PCR primers were designed based on human ATP2A1 sequences (GenBank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799. Primer Sequences: Primers derived from the porcine sequences were as follows: Set A: forward, 5'-GTC TCA GCC AGC CAA TCC CT-3' and reverse, 5'-AAG GAA ATG CAT GCG GCC AG-3'; Set B: forward, 5'-GGG CAC CAA CAT TGC AGC C-3' and reverse, 5'-CTT GCA GAC AGA CAT CTG GTT-3'.