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https://hdl.handle.net/1959.11/31131
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Feyera, T | en |
dc.contributor.author | Ruhnke, I | en |
dc.contributor.author | Sharpe, B | en |
dc.contributor.author | Elliott, T | en |
dc.contributor.author | Campbell, D L M | en |
dc.contributor.author | Walkden-Brown, S W | en |
dc.date.accessioned | 2021-07-27T03:53:44Z | - |
dc.date.available | 2021-07-27T03:53:44Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Helminthology, v.94, p. 1-9 | en |
dc.identifier.issn | 1475-2697 | en |
dc.identifier.issn | 0022-149X | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/31131 | - |
dc.description.abstract | Eggs oviposited by <i>Ascaridia galli</i> females in artificial media are commonly used as a source of infective material. We investigated the rate of egg production by cultured mature females (<i>n</i> = 223), and changes in egg viability under different storage and incubation conditions. Eggs recovered after 1, 2 or 3 days of culture were subjected to either (1) storage in water at 4°C (1, 4 or 8 weeks) followed by incubation in 0.1 N H<sub>2</sub>SO<sub>4</sub> at 26°C (2, 4 or 6 weeks); or (2) prolonged storage at 4°C (up to 14 weeks). Egg development and viability was assessed by morphology coupled with a viability dye exclusion test of hatched larvae. Of the 6,044 eggs recovered per mature female 49.2, 38.5 and 12.3% were recovered on days 1, 2 and 3 of worm incubation respectively with similar initial viability (≥99%) between days. Eggs recovered on different days had only minor differences in viability after storage. The prolonged storage period at 4°C significantly affected both viability and embryonation ability resulting in decline in viability of 5.7–6.2% per week. A smaller but significant decline in egg (2.0%) and hatched larval (1.4%) viability per week of incubation at 26°C was also observed. We conclude that storage and incubation conditions, not the day of egg recovery, are the main factors affecting <i>A. galli</i> egg viability. Our findings indicate that under aerobic conditions storage at 26°C may be preferable to 4°C whereas other studies indicate that under anaerobic conditions storage at 4°C is preferable. | en |
dc.language | en | en |
dc.publisher | Cambridge University Press | en |
dc.relation.ispartof | Journal of Helminthology | en |
dc.title | Viability and development of Ascaridia galli eggs recovered in artificial media followed by storage under different conditions | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1017/S0022149X2000084X | en |
dc.identifier.pmid | 33046154 | en |
local.contributor.firstname | T | en |
local.contributor.firstname | I | en |
local.contributor.firstname | B | en |
local.contributor.firstname | T | en |
local.contributor.firstname | D L M | en |
local.contributor.firstname | S W | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | Animal Science, School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | tdewo@myune.edu.au | en |
local.profile.email | iruhnke@une.edu.au | en |
local.profile.email | tellio22@une.edu.au | en |
local.profile.email | dcampb38@une.edu.au | en |
local.profile.email | swalkden@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | United Kingdom | en |
local.identifier.runningnumber | e199 | en |
local.format.startpage | 1 | en |
local.format.endpage | 9 | en |
local.identifier.scopusid | 85092886918 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 94 | en |
local.contributor.lastname | Feyera | en |
local.contributor.lastname | Ruhnke | en |
local.contributor.lastname | Sharpe | en |
local.contributor.lastname | Elliott | en |
local.contributor.lastname | Campbell | en |
local.contributor.lastname | Walkden-Brown | en |
dc.identifier.staff | une-id:tdewo | en |
dc.identifier.staff | une-id:iruhnke | en |
dc.identifier.staff | une-id:tellio22 | en |
dc.identifier.staff | une-id:dcampb38 | en |
dc.identifier.staff | une-id:swalkden | en |
local.profile.orcid | 0000-0001-5423-9306 | en |
local.profile.orcid | 0000-0002-0638-5533 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:1959.11/31131 | en |
local.date.onlineversion | 2020-10-13 | - |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Viability and development of Ascaridia galli eggs recovered in artificial media followed by storage under different conditions | en |
local.relation.fundingsourcenote | This research was funded by the Australian Eggs (Project 1BS003). Teka Feyera was supported by a University of New England postgraduate scholarship. | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Feyera, T | en |
local.search.author | Ruhnke, I | en |
local.search.author | Sharpe, B | en |
local.search.author | Elliott, T | en |
local.search.author | Campbell, D L M | en |
local.search.author | Walkden-Brown, S W | en |
local.uneassociation | Yes | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.identifier.wosid | 000576857600001 | en |
local.year.available | 2020 | en |
local.year.published | 2020 | en |
local.fileurl.closedpublished | https://rune.une.edu.au/web/retrieve/3f99393d-3186-4eea-8267-21d8537de53d | en |
local.subject.for2020 | 300303 Animal nutrition | en |
local.subject.seo2020 | 100411 Poultry | en |
dc.notification.token | d0fa2a2c-28d4-4b48-8838-75666d73b620 | en |
Appears in Collections: | Journal Article School of Environmental and Rural Science |
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