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https://hdl.handle.net/1959.11/30664
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DC Field | Value | Language |
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dc.contributor.author | Nonhebel, Heather M | en |
dc.contributor.author | Griffin, Karina | en |
dc.date.accessioned | 2021-05-26T07:40:03Z | - |
dc.date.available | 2021-05-26T07:40:03Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Functional Plant Biology, 47(8), p. 716-726 | en |
dc.identifier.issn | 1445-4416 | en |
dc.identifier.issn | 1445-4408 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/30664 | - |
dc.description.abstract | Current understanding of the role of plant hormones during cereal grain filling is confounded by contradictory reports on hormone production that is based on poor methodology. We report here on the accurate measurement of indole-3-acetic acid (IAA) and abscisic acid (ABA) by combined liquid chromatography-tandem mass spectrometry in multiple reaction-monitoring mode with heavy isotope labelled internal standards. ABA and IAA contents of superior versus inferior rice grains (ABA maxima 159 ng g<sup>–1</sup> FW and 109 ng g<sup>–1</sup> FW, IAA maxima 2 µg g<sup>–1</sup> FW and 1.7 µg g<sup>–1</sup> FW respectively) correlated with the expression of biosynthetic genes and with grain fill. Results confirm that grain ABA is produced primarily by OsNCED2(5), but suggest that ABA import and metabolism also play important roles in ABA regulation. The IAA content of grains is primarily influenced by <I>OsYUC9</I> and <I>OsYUC11</I>. However, the distinct expression profile of <I>OsYUC12</I> suggests a specific role for IAA produced by this enzyme. Co-expression of <I>OsYUC12</I> with <I>OsIAA29</I> indicates their involvement in a common signalling pathway. Co-expression and cis-element analysis identified several aleurone-specific transcriptional regulators as well as glutelin as strong candidates for detailed investigation for direct regulation by the auxin-signalling pathway. | en |
dc.language | en | en |
dc.publisher | CSIRO Publishing | en |
dc.relation.ispartof | Functional Plant Biology | en |
dc.title | Production and roles of IAA and ABA during development of superior and inferior rice grains | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1071/FP19291 | en |
local.contributor.firstname | Heather M | en |
local.contributor.firstname | Karina | en |
local.profile.school | School of Science and Technology | en |
local.profile.email | hnonheb2@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | Australia | en |
local.format.startpage | 716 | en |
local.format.endpage | 726 | en |
local.identifier.scopusid | 85085759092 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 47 | en |
local.identifier.issue | 8 | en |
local.contributor.lastname | Nonhebel | en |
local.contributor.lastname | Griffin | en |
dc.identifier.staff | une-id:hnonheb2 | en |
local.profile.orcid | 0000-0001-9055-3095 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:1959.11/30664 | en |
local.date.onlineversion | 2020-05-22 | - |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Production and roles of IAA and ABA during development of superior and inferior rice grains | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Nonhebel, Heather M | en |
local.search.author | Griffin, Karina | en |
local.uneassociation | Yes | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.year.available | 2020 | en |
local.year.published | 2020 | en |
local.fileurl.closedpublished | https://rune.une.edu.au/web/retrieve/30ababaa-4e63-4540-aec5-96e10da001a7 | en |
local.subject.for2020 | 300404 Crop and pasture biochemistry and physiology | en |
local.subject.seo2020 | 260308 Rice | en |
Appears in Collections: | Journal Article School of Science and Technology |
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