Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/27047
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fan, Jinghui | en |
dc.contributor.author | Gerber, Priscilla F | en |
dc.contributor.author | Cubas Atienzar, Ana | en |
dc.contributor.author | Eppink, Lysan | en |
dc.contributor.author | Wang, Chong | en |
dc.contributor.author | Opriessnig, Tanja | en |
dc.date.accessioned | 2019-05-31T06:12:33Z | - |
dc.date.available | 2019-05-31T06:12:33Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Veterinary Record, 185(1), p. 1-6 | en |
dc.identifier.issn | 2042-7670 | en |
dc.identifier.issn | 0042-4900 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/27047 | - |
dc.description.abstract | In the UK, approximately 40 per cent of the pig breeding herds are outdoors. To monitor their porcine reproductive and respiratory syndrome virus (PRRSV) status, blood is collected commonly from piglets around weaning. Sample collection in British outdoor pigs often occurs during the early morning hours when the piglets tend to accumulate inside sheltered areas. For practical reasons, dry cotton swabs are occasionally used for blood collection and stored at room temperature until arrival in the laboratory. Detection of PRRSV RNA is a function of viral concentration, sample type and storage condition. To evaluate a possible impact of the sampling protocol on PRRSV1 detection, experimentally spiked blood samples using three dilutions of a representative PRRSV1 strain were prepared. In addition, blood samples from pigs naturally infected with PRRSV were obtained from a PRRSV-positive British herd. Spiked blood and blood from infected pigs were used to obtain sera, dry or wet (immersed in saline) polyester or cotton swabs and FTA cards. The different samples were stored for 24 hours, 48 hours or 7 days at 4°C or 20°C and tested by a real-time reverse transcriptase PRRSV PCR assay. Under the study conditions, the best matrix was serum (96.7 per cent), followed by wet swabs (78 per cent), dry swabs (61.3 per cent) and FTA cards (51 per cent). Polyester swabs (76 per cent) showed a better performance than cotton swabs (63.3 per cent). The reduction in sensitivity obtained for swabs and FTA cards was particularly high at low viral concentrations. The results indicate that wet polyester swabs should be used whenever possible. | en |
dc.language | en | en |
dc.publisher | BMJ Group | en |
dc.relation.ispartof | Veterinary Record | en |
dc.rights | Attribution-NonCommercial 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.title | Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1136/vr.105312 | en |
dc.identifier.pmid | 31040221 | en |
dcterms.accessRights | UNE Green | en |
local.contributor.firstname | Jinghui | en |
local.contributor.firstname | Priscilla F | en |
local.contributor.firstname | Ana | en |
local.contributor.firstname | Lysan | en |
local.contributor.firstname | Chong | en |
local.contributor.firstname | Tanja | en |
local.subject.for2008 | 070712 Veterinary Virology | en |
local.subject.seo2008 | 830308 Pigs | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | pgerber2@une.edu.au | en |
local.output.category | C1 | en |
local.grant.number | BB/J004324/1 | en |
local.grant.number | BBS/E/D/20241864 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | United Kingdom | en |
local.format.startpage | 1 | en |
local.format.endpage | 6 | en |
local.identifier.scopusid | 85065238426 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 185 | en |
local.identifier.issue | 1 | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Fan | en |
local.contributor.lastname | Gerber | en |
local.contributor.lastname | Cubas Atienzar | en |
local.contributor.lastname | Eppink | en |
local.contributor.lastname | Wang | en |
local.contributor.lastname | Opriessnig | en |
dc.identifier.staff | une-id:pgerber2 | en |
local.profile.orcid | 0000-0002-8343-8299 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:1959.11/27047 | en |
local.date.onlineversion | 2019-04-30 | - |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK | en |
local.relation.fundingsourcenote | Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Fan, Jinghui | en |
local.search.author | Gerber, Priscilla F | en |
local.search.author | Cubas Atienzar, Ana | en |
local.search.author | Eppink, Lysan | en |
local.search.author | Wang, Chong | en |
local.search.author | Opriessnig, Tanja | en |
local.istranslated | No | en |
local.uneassociation | Yes | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.identifier.wosid | 000474628600017 | en |
local.year.available | 2019 | en |
local.year.published | 2019 | en |
local.fileurl.closedpublished | https://rune.une.edu.au/web/retrieve/317db024-bab5-4886-b145-0d056a858cca | en |
local.subject.for2020 | 300914 Veterinary virology | en |
local.subject.seo2020 | 100410 Pigs | en |
Appears in Collections: | Journal Article School of Environmental and Rural Science |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
open/PorcineFreitasGerber2020JournalArticlePostPeerReview.pdf | Open access version | 407.19 kB | Adobe PDF Download Adobe | View/Open |
This item is licensed under a Creative Commons License