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https://hdl.handle.net/1959.11/21270
Title: | Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2 | Contributor(s): | Gimenez-Lirola, Luis G (author); Gerber, Priscilla Freitas (author) ; Rowland, Raymond R (author); Halbur, Patrick G (author); Huang, Yao-Wei (author); Meng, Xiang-Jin (author); Opriessnig, Tanja (author) | Publication Date: | 2014 | DOI: | 10.1016/j.rvsc.2014.02.014 | Handle Link: | https://hdl.handle.net/1959.11/21270 | Abstract: | A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem. | Publication Type: | Journal Article | Source of Publication: | Research in Veterinary Science, 96(3), p. 543-550 | Publisher: | Elsevier Ltd | Place of Publication: | United Kingdom | ISSN: | 1532-2661 0034-5288 |
Fields of Research (FoR) 2008: | 070712 Veterinary Virology 070703 Veterinary Diagnosis and Diagnostics 070706 Veterinary Medicine |
Fields of Research (FoR) 2020: | 300914 Veterinary virology 300904 Veterinary diagnosis and diagnostics 300907 Veterinary medicine (excl. urology) |
Socio-Economic Objective (SEO) 2008: | 830308 Pigs | Socio-Economic Objective (SEO) 2020: | 100410 Pigs | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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