Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/20677
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DC Field | Value | Language |
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dc.contributor.author | Huma, Zil E | en |
dc.contributor.author | Ludeman, Justin P | en |
dc.contributor.author | Wilkinson, Brendan | en |
dc.contributor.author | Payne, Richard J | en |
dc.contributor.author | Stone, Martin J | en |
dc.date.accessioned | 2017-05-05T15:40:00Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Chemical Science, 5(7), p. 2783-2788 | en |
dc.identifier.issn | 2041-6539 | en |
dc.identifier.issn | 2041-6520 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/20677 | - |
dc.description.abstract | We describe a general approach for analysis of 2D NMR spectra to evaluate the cooperativity of ligand binding and protein dimerization in coupled systems. The approach is applicable to systems in which NMR spectra display separate resonances for monomeric and dimeric species but each resonance shifts in response to ligand binding. Three experimental parameters (monomer chemical shift, dimer chemical shift and relative monomer-dimer peak intensity) are fitted globally, as a function of ligand concentration, to yield equilibrium constants for dimerization, monomer-ligand binding and dimer-ligand binding as well as the cooperativity between ligand binding and dimerization. We have applied the approach to characterise a system in which dimerization of the chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2) is coupled to binding of peptides derived from the chemokine receptor CCR2. The global fitting approach allowed evaluation of cooperativity with higher precision than is possible by alternative methods. | en |
dc.language | en | en |
dc.publisher | Royal Society of Chemistry | en |
dc.relation.ispartof | Chemical Science | en |
dc.title | NMR characterization of cooperativity: fast ligand binding coupled to slow protein dimerization | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1039/c4sc00131a | en |
dcterms.accessRights | Gold | en |
dc.subject.keywords | Biomolecular Modelling and Design | en |
dc.subject.keywords | Proteins and Peptides | en |
dc.subject.keywords | Characterisation of Biological Macromolecules | en |
local.contributor.firstname | Zil E | en |
local.contributor.firstname | Justin P | en |
local.contributor.firstname | Brendan | en |
local.contributor.firstname | Richard J | en |
local.contributor.firstname | Martin J | en |
local.subject.for2008 | 030403 Characterisation of Biological Macromolecules | en |
local.subject.for2008 | 030406 Proteins and Peptides | en |
local.subject.for2008 | 030402 Biomolecular Modelling and Design | en |
local.subject.seo2008 | 970103 Expanding Knowledge in the Chemical Sciences | en |
local.subject.seo2008 | 929999 Health not elsewhere classified | en |
local.subject.seo2008 | 970106 Expanding Knowledge in the Biological Sciences | en |
local.profile.school | School of Science and Technology | en |
local.profile.email | bwilkin7@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20170323-112917 | en |
local.publisher.place | United Kingdom | en |
local.format.startpage | 2783 | en |
local.format.endpage | 2788 | en |
local.identifier.scopusid | 84901818669 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 5 | en |
local.identifier.issue | 7 | en |
local.title.subtitle | fast ligand binding coupled to slow protein dimerization | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Huma | en |
local.contributor.lastname | Ludeman | en |
local.contributor.lastname | Wilkinson | en |
local.contributor.lastname | Payne | en |
local.contributor.lastname | Stone | en |
dc.identifier.staff | une-id:bwilkin7 | en |
local.profile.orcid | 0000-0003-1866-6540 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:20870 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | NMR characterization of cooperativity | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.relation.grantdescription | ARC/DE130101673 | en |
local.search.author | Huma, Zil E | en |
local.search.author | Ludeman, Justin P | en |
local.search.author | Wilkinson, Brendan | en |
local.search.author | Payne, Richard J | en |
local.search.author | Stone, Martin J | en |
local.uneassociation | Unknown | en |
local.year.published | 2014 | en |
local.subject.for2020 | 340402 Biomolecular modelling and design | en |
local.subject.for2020 | 340407 Proteins and peptides | en |
local.subject.for2020 | 340403 Characterisation of biological macromolecules | en |
local.subject.seo2020 | 280105 Expanding knowledge in the chemical sciences | en |
local.subject.seo2020 | 280102 Expanding knowledge in the biological sciences | en |
local.codeupdate.date | 2022-02-13T16:40:33.469 | en |
local.codeupdate.eperson | bwilkin7@une.edu.au | en |
local.codeupdate.finalised | true | en |
local.original.for2020 | 340403 Characterisation of biological macromolecules | en |
local.original.for2020 | 340407 Proteins and peptides | en |
local.original.for2020 | 340402 Biomolecular modelling and design | en |
local.original.seo2020 | 280105 Expanding knowledge in the chemical sciences | en |
local.original.seo2020 | undefined | en |
local.original.seo2020 | 280102 Expanding knowledge in the biological sciences | en |
Appears in Collections: | Journal Article School of Science and Technology |
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