Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/19656
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dc.contributor.authorBarnett, Marken
dc.contributor.authorForster, Nicolaen
dc.contributor.authorRay, G Aen
dc.contributor.authorLi, Len
dc.contributor.authorGuppy, Christopheren
dc.contributor.authorHegarty, Rogeren
dc.date.accessioned2016-11-24T12:09:00Z-
dc.date.issued2016-
dc.identifier.citationAnimal Feed Science and Technology, v.212, p. 35-41en
dc.identifier.issn1873-2216en
dc.identifier.issn0377-8401en
dc.identifier.urihttps://hdl.handle.net/1959.11/19656-
dc.description.abstractConcentration of indigestible markers in feces is routinely used to estimate the digestibility or rate of passage of feed through the gut. Current procedure for measuring fecal marker concentrations is an acid digest before analysis using inductively coupled plasma optical emission spectroscopy (ICP). Portable X-ray fluorescence (pXRF) spectroscopy uses short wavelength X-rays to excite sample material and generate characteristic elemental emissions proportional to the concentration of the element present in ground fecal material. The aim of this study was to measure fecal concentrations of Co, Cr, Ti, and Yb digesta marker by both ICP and pXRF and determine any correlation between the two methods, thereby assessing the potential of using pXRF as a marker analysis method. Silica (Si) concentrations in fecal and feed samples were also measured using both methods. Cattle and sheep feces and various feed samples collect from four separate studies were analyzed for concentrations of Co (CoEDTA), Cr (Cr mordanted fibre), Ti (TiO2), Yb (Yb(III)acetate), and Si using ICP and pXRF. Regression analysis used to assess the relationship between ICP and pXRF determined fecal concentrations indicated strong linear relationships (P<0.01) between pXRF and ICP estimates of Co (r2=1.00), Cr (r2=0.95), Ti (r2=0.97), Yb (r2=0.94), and Si (r2=0.88). All curves were validated using a second set of independent fecal samples. A full set of total collection fecal samples for six sheep were also analyzed by pXRF for Co and Cr (digesta kinetics), and Si (digestion) marker concentrations. These fecal Co and Cr concentrations were used to estimate digesta mean retention times (MRT) and compared to estimated digesta MRT from the same sheep using Co and Cr marker concentrations determined by ICP analysis. No differences in estimated MRT were found when using either analytical method. Silica concentrations from the same fecal samples as determined by pXRF were used to determine the rate of DMD for each animal. When compared to apparent DMD for the same animals as determined using feed DM intake and fecal DM output collected during the total collection, no differences were again found using either analytical method. The results indicate that pXRF can be used as an alternative to standard ICP procedure, obviating the need for acid digestion, reducing cost and time; and may also offer an alternative analytical method for determining livestock DM digestibility.en
dc.languageenen
dc.publisherElsevier BVen
dc.relation.ispartofAnimal Feed Science and Technologyen
dc.titleUsing portable X-ray fluorescence (pXRF) to determine fecal concentrations of non-absorbable digesta kinetic and digestibility markers in sheep and cattleen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.anifeedsci.2015.12.015en
dc.subject.keywordsAnimal Nutritionen
local.contributor.firstnameMarken
local.contributor.firstnameNicolaen
local.contributor.firstnameG Aen
local.contributor.firstnameLen
local.contributor.firstnameChristopheren
local.contributor.firstnameRogeren
local.subject.for2008070204 Animal Nutritionen
local.subject.seo2008830406 Sown Pastures (excl. Lucerne)en
local.subject.seo2008830403 Native and Residual Pasturesen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolAnimal Genetics and Breeding Uniten
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailmbarnet3@une.edu.auen
local.profile.emaillli4@une.edu.auen
local.profile.emailcguppy@une.edu.auen
local.profile.emailrhegart3@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-chute-20161112-192430en
local.publisher.placeNetherlandsen
local.format.startpage35en
local.format.endpage41en
local.identifier.scopusid84955172517en
local.peerreviewedYesen
local.identifier.volume212en
local.contributor.lastnameBarnetten
local.contributor.lastnameForsteren
local.contributor.lastnameRayen
local.contributor.lastnameLien
local.contributor.lastnameGuppyen
local.contributor.lastnameHegartyen
dc.identifier.staffune-id:mbarnet3en
dc.identifier.staffune-id:nforste3en
dc.identifier.staffune-id:lli4en
dc.identifier.staffune-id:cguppyen
dc.identifier.staffune-id:rhegart3en
local.profile.orcid0000-0001-6306-7884en
local.profile.orcid0000-0002-3601-9729en
local.profile.orcid0000-0001-7274-607Xen
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:19846en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleUsing portable X-ray fluorescence (pXRF) to determine fecal concentrations of non-absorbable digesta kinetic and digestibility markers in sheep and cattleen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorBarnett, Marken
local.search.authorForster, Nicolaen
local.search.authorRay, G Aen
local.search.authorLi, Len
local.search.authorGuppy, Christopheren
local.search.authorHegarty, Rogeren
local.uneassociationUnknownen
local.identifier.wosid000369461300004en
local.year.published2016en
local.fileurl.closedpublishedhttps://rune.une.edu.au/web/retrieve/5cb9395d-ebba-4fd4-a2f4-148079f8bb54en
local.subject.for2020300303 Animal nutritionen
local.subject.seo2020100505 Sown pastures (excl. lucerne)en
local.subject.seo2020100503 Native and residual pasturesen
Appears in Collections:Animal Genetics and Breeding Unit (AGBU)
Journal Article
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