Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/17282
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dc.contributor.authorMacharia, Isaacen
dc.contributor.authorBackhouse, Daviden
dc.contributor.authorAteka, Elijah Miindaen
dc.contributor.authorWu, Shubiaoen
dc.contributor.authorHarvey, Jaggeren
dc.contributor.authorNjahira, Mosesen
dc.contributor.authorSkilton, Robert Aen
dc.date.accessioned2015-05-12T10:45:00Z-
dc.date.issued2015-
dc.identifier.citationAnnals of Applied Biology, 166(3), p. 520-529en
dc.identifier.issn1744-7348en
dc.identifier.issn0003-4746en
dc.identifier.urihttps://hdl.handle.net/1959.11/17282-
dc.description.abstract'Tomato spotted wilt virus' (TSWV) affects the production of many horticultural crops worldwide. It was first reported from Kenya in 1999. The occurrence, distribution and genetic diversity of TSWV were evaluated in four tomato production areas in Kenya a decade after this incursion. The awareness of TSWV and its vectors among farmers was assessed through a questionnaire while plant samples including tomato leaves and fruit were collected from diseased and non-diseased plants. The samples were assayed for TSWV using ELISA and reverse transcriptase-polymerase chain reaction, and the resulting positive samples were sequenced. There was less awareness of the virus, its vectors and alternate hosts among farmers, despite the occurrence of the disease for over a decade. A total of 89 of 408 tomato samples tested positive for TSWV. Most of the positive samples came from Nakuru, where the virus was first detected, with only a single positive sample being obtained from Loitokitok and none from Bungoma or Kirinyaga. A phylogenetic analysis based on partial nucleocapsid (N) protein gene sequences suggested that the Kenyan isolates formed a single subgroup nested within a cluster of isolates that came predominantly from Europe. This indicated a single introduction that had undergone limited diversification. The study revealed that the disease has persisted in the area to which it was first introduced but has had very limited dispersal to other areas.en
dc.languageenen
dc.publisherWiley-Blackwell Publishing Ltden
dc.relation.ispartofAnnals of Applied Biologyen
dc.titleDistribution and genetic diversity of 'Tomato spotted wilt virus' following an incursion into Kenyaen
dc.typeJournal Articleen
dc.identifier.doi10.1111/aab.12201en
dc.subject.keywordsPlant Pathologyen
dc.subject.keywordsCrop and Pasture Protection (Pests, Diseases and Weeds)en
dc.subject.keywordsHorticultural Crop Protection (Pests, Diseases and Weeds)en
local.contributor.firstnameIsaacen
local.contributor.firstnameDaviden
local.contributor.firstnameElijah Miindaen
local.contributor.firstnameShubiaoen
local.contributor.firstnameJaggeren
local.contributor.firstnameMosesen
local.contributor.firstnameRobert Aen
local.subject.for2008060704 Plant Pathologyen
local.subject.for2008070308 Crop and Pasture Protection (Pests, Diseases and Weeds)en
local.subject.for2008070603 Horticultural Crop Protection (Pests, Diseases and Weeds)en
local.subject.seo2008820215 Vegetablesen
local.profile.schoolEnvironmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolEnvironmental and Rural Scienceen
local.profile.schoolEnvironmental and Rural Scienceen
local.profile.schoolEnvironmental and Rural Scienceen
local.profile.emailimachari@une.edu.auen
local.profile.emaildbackhou@une.edu.auen
local.profile.emailemateka@yahoo.comen
local.profile.emailswu3@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20150504-153023en
local.publisher.placeUnited Kingdomen
local.format.startpage520en
local.format.endpage529en
local.identifier.scopusid84926526935en
local.peerreviewedYesen
local.identifier.volume166en
local.identifier.issue3en
local.contributor.lastnameMachariaen
local.contributor.lastnameBackhouseen
local.contributor.lastnameAtekaen
local.contributor.lastnameWuen
local.contributor.lastnameHarveyen
local.contributor.lastnameNjahiraen
local.contributor.lastnameSkiltonen
dc.identifier.staffune-id:imacharien
dc.identifier.staffune-id:dbackhouen
dc.identifier.staffune-id:eatekaen
dc.identifier.staffune-id:swu3en
local.profile.orcid0000-0003-0663-6002en
local.profile.orcid0000-0002-1790-6015en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:17496en
local.identifier.handlehttps://hdl.handle.net/1959.11/17282en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleDistribution and genetic diversity of 'Tomato spotted wilt virus' following an incursion into Kenyaen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorMacharia, Isaacen
local.search.authorBackhouse, Daviden
local.search.authorAteka, Elijah Miindaen
local.search.authorWu, Shubiaoen
local.search.authorHarvey, Jaggeren
local.search.authorNjahira, Mosesen
local.search.authorSkilton, Robert Aen
local.uneassociationUnknownen
local.identifier.wosid000352625400014en
local.year.published2015en
local.subject.for2020310805 Plant pathologyen
local.subject.for2020300409 Crop and pasture protection (incl. pests, diseases and weeds)en
local.subject.for2020300804 Horticultural crop protection (incl. pests, diseases and weeds)en
local.subject.seo2020260512 Protected vegetable cropsen
local.subject.seo2020260505 Field grown vegetable cropsen
Appears in Collections:Journal Article
School of Environmental and Rural Science
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