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https://hdl.handle.net/1959.11/16992
Title: | Replication kinetics, shedding, transmission and protective efficacy of Rispens/CVI988 vaccine virus in single and combined infections with very virulent Marek's disease virus | Contributor(s): | Islam, Tanzila (author); Walkden-Brown, Steve (supervisor) ; Renz, Katrin (supervisor); Islam, A Fakhrul (supervisor) | Conferred Date: | 2014 | Copyright Date: | 2013 | Open Access: | Yes | Handle Link: | https://hdl.handle.net/1959.11/16992 | Abstract: | The attenuated MDV-1 Rispens/CVI988 vaccine is widely used to vaccinate chickens worldwide and is the most effective current vaccine against MD. Two experiments were designed to investigate transmission of the Rispens/CVI988 vaccine virus (Rispens/CVI988) between chickens, the viral kinetics and shedding profile of Rispens/CVI988 and very virulent MDV (vvMDV, isolate 02LAR) in single and mixed infections and the effect of vaccination to challenge interval on the protection provided by Rispens/CVI988 against vvMDV challenge. Experiment 1 used 70 specific pathogen free (SPF) chickens in four climate controlled rooms. In three rooms, 10 chickens were vaccinated with one of the three commercial Rispens/CVI988 vaccines at day old and left in contact with 10 unvaccinated chickens. The fourth room held 10 unvaccinated control birds. As determined by MDV-specific qPCR of weekly room dust, PBL and feather samples the commercially available Rispens/CVI988 vaccine virus strains are shed in significant quantities and transmit effectively to in-contact chickens. Experiment 2 used 600 commercial ISA Brown layers of the same age (day old) in 24 isolators. Chickens were vaccinated with Rispens/CVI988 (3200 pfu) and/or challenged with the 02LAR (400 pfu) on days 0, 5, 10 post hatching providing vaccination to challenge intervals (VCI) of -10, -5, 0, 5, 10 days with the negative values indicating challenge prior to vaccination. As determined by MDV-specific qPCR able to differentiate between the viruses, vaccination with Rispens/CVI988 greatly reduced the viral load of vvMDV in PBL and feather cells but only if birds were vaccinated prior challenge. | Publication Type: | Thesis Doctoral | Fields of Research (FoR) 2008: | 070710 Veterinary Pharmacology 070205 Animal Protection (Pests and Pathogens) |
Fields of Research (FoR) 2020: | 300911 Veterinary pharmacology 300304 Animal protection (incl. pests and pathogens) |
Socio-Economic Objective (SEO) 2008: | 830309 Poultry | Socio-Economic Objective (SEO) 2020: | 100411 Poultry | Rights Statement: | Copyright 2013 - Tanzila Islam | HERDC Category Description: | T2 Thesis - Doctorate by Research |
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Appears in Collections: | School of Environmental and Rural Science Thesis Doctoral |
Files in This Item:
File | Description | Size | Format | |
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open/MARCXML.xml | MARCXML.xml | 3.67 kB | Unknown | View/Open |
open/SOURCE06.pdf | Thesis, part 3 | 7.42 MB | Adobe PDF Download Adobe | View/Open |
open/SOURCE05.pdf | Thesis, part 2 | 1.44 MB | Adobe PDF Download Adobe | View/Open |
open/SOURCE07.pdf | Thesis, part 4 | 357.78 kB | Adobe PDF Download Adobe | View/Open |
open/SOURCE04.pdf | Thesis, part 1 | 2.75 MB | Adobe PDF Download Adobe | View/Open |
open/SOURCE03.pdf | Abstract | 282.58 kB | Adobe PDF Download Adobe | View/Open |
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