Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/16579
Title: Profilin-1 Overexpression in MDA-MB-231 Breast Cancer Cells Is Associated with Alterations in Proteomics Biomarkers of Cell Proliferation, Survival, and Motility as Revealed by Global Proteomics Analyses
Contributor(s): Coumans-Moens, Joelle  (author)orcid ; Gau, David (author); Poljak, Anne (author); Wasinger, Valerie (author); Roy, Partha (author); Moens, Pierre  (author)orcid 
Publication Date: 2014
Open Access: Yes
DOI: 10.1089/omi.2014.0075Open Access Link
Handle Link: https://hdl.handle.net/1959.11/16579
Open Access Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253143Open Access Link
Abstract: Despite early screening programs and new therapeutic strategies, metastatic breast cancer is still the leading cause of cancer death in women in industrialized countries and regions. There is a need for novel biomarkers of susceptibility, progression, and therapeutic response. Global analyses or systems science approaches with omics technologies offer concrete ways forward in biomarker discovery for breast cancer. Previous studies have shown that expression of profilin-1 (PFN1), a ubiquitously expressed actin-binding protein, is downregulated in invasive and metastatic breast cancer. It has also been reported that PFN1 overexpression can suppress tumorigenic ability and motility/invasiveness of breast cancer cells. To obtain insights into the underlying molecular mechanisms of how elevating PFN1 level induces these phenotypic changes in breast cancer cells, we investigated the alteration in global protein expression profiles of breast cancer cells upon stable overexpression of PFN1 by a combination of three different proteome analysis methods (2-DE, iTRAQ, label-free). Using MDA-MB-231 as a model breast cancer cell line, we provide evidence that PFN1 overexpression is associated with alterations in the expression of proteins that have been functionally linked to cell proliferation (FKPB1A, HDGF, MIF, PRDX1, TXNRD1, LGALS1, STMN1, LASP1, S100A11, S100A6), survival (HSPE1, HSPB1, HSPD1, HSPA5 and PPIA, YWHAZ, CFL1, NME1) and motility (CFL1, CORO1B, PFN2, PLS3, FLNA, FLNB, NME2, ARHGDIB). In view of the pleotropic effects of PFN1 overexpression in breast cancer cells as suggested by these new findings, we propose that PFN1-induced phenotypic changes in cancer cells involve multiple mechanisms. Our data reported here might also offer innovative strategies for identification and validation of novel therapeutic targets and companion diagnostics for persons with, or susceptibility to, breast cancer.
Publication Type: Journal Article
Source of Publication: OMICS: A Journal of Integrative Biology, 18(12), p. 778-791
Publisher: Mary Ann Liebert Inc
Place of Publication: United States of America
ISSN: 1557-8100
Field of Research (FOR): 060103 Cell Development, Proliferation and Death
060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)
060104 Cell Metabolism
Socio-Economic Outcome Codes: 920102 Cancer and Related Disorders
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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