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https://hdl.handle.net/1959.11/13981
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DC Field | Value | Language |
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dc.contributor.author | Zheng, Lingling | en |
dc.contributor.author | Dang, Zhenghua | en |
dc.contributor.author | Li, Haoyu | en |
dc.contributor.author | Zhang, Huirong | en |
dc.contributor.author | Wu, Shubiao | en |
dc.contributor.author | Wang, Yingchun | en |
dc.date.accessioned | 2014-02-10T17:05:00Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Molecular Biology Reports, 41(1), p. 563-572 | en |
dc.identifier.issn | 1573-4978 | en |
dc.identifier.issn | 0301-4851 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/13981 | - |
dc.description.abstract | Several functional and regulatory proteins play important roles in controlling plant stress tolerance. Proline (Pro) is one of the most accumulated osmolytes correlated with tolerance to stresses. Δ¹-Pyrroline-5-carboxylate synthetase (P5CS) is a rate-limiting enzyme in Pro biosynthesis. In the present study, we isolated the cDNA for a P5CS gene ('NtP5CS') from the halophyte 'Nitraria tangutorum'. Phylogenetic analysis and subcellular localization analysis of NtP5CS-GFP protein in onion cells showed that 'NtP5CS' was a new 'P5CS' gene and was involved in Pro synthesis in 'N. tangutorum'. Expression of the 'NtP5CS' gene was induced by salt stress, dehydration, and high and low temperatures. 'Escherichia coli' overexpressing 'AtP5CS' or 'NtP5CS' exhibited better growth in all treatments, including high salinity, high alkalinity, dehydration, osmotic, heat and cold stresses. Additionally, 'NtP5CS' recombinant 'E. coli' cells grew better than did 'AtP5CS' recombinant cells in response to abiotic stresses. Our data demonstrate that the 'P5CS' from a halophytic species functions more efficiently than its homologue from a glycophytic species in improving the stress tolerance of 'E. coli'. | en |
dc.language | en | en |
dc.publisher | Springer Netherlands | en |
dc.relation.ispartof | Molecular Biology Reports | en |
dc.title | Isolation and characterization of a Δ¹-pyrroline-5-carboxylate synthetase (NtP5CS) from 'Nitraria tangutorum' Bobr. and functional comparison with its 'Arabidopsis' homologue | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1007/s11033-013-2893-8 | en |
dc.subject.keywords | Plant Physiology | en |
local.contributor.firstname | Lingling | en |
local.contributor.firstname | Zhenghua | en |
local.contributor.firstname | Haoyu | en |
local.contributor.firstname | Huirong | en |
local.contributor.firstname | Shubiao | en |
local.contributor.firstname | Yingchun | en |
local.subject.for2008 | 060705 Plant Physiology | en |
local.subject.seo2008 | 829899 Environmentally Sustainable Plant Production not elsewhere classified | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | swu3@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20131214-151218 | en |
local.publisher.place | Netherlands | en |
local.format.startpage | 563 | en |
local.format.endpage | 572 | en |
local.identifier.scopusid | 84891956175 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 41 | en |
local.identifier.issue | 1 | en |
local.contributor.lastname | Zheng | en |
local.contributor.lastname | Dang | en |
local.contributor.lastname | Li | en |
local.contributor.lastname | Zhang | en |
local.contributor.lastname | Wu | en |
local.contributor.lastname | Wang | en |
dc.identifier.staff | une-id:swu3 | en |
local.profile.orcid | 0000-0002-1790-6015 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:14194 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Isolation and characterization of a Δ¹-pyrroline-5-carboxylate synthetase (NtP5CS) from 'Nitraria tangutorum' Bobr. and functional comparison with its 'Arabidopsis' homologue | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Zheng, Lingling | en |
local.search.author | Dang, Zhenghua | en |
local.search.author | Li, Haoyu | en |
local.search.author | Zhang, Huirong | en |
local.search.author | Wu, Shubiao | en |
local.search.author | Wang, Yingchun | en |
local.uneassociation | Unknown | en |
local.identifier.wosid | 000329100500061 | en |
local.year.published | 2014 | en |
local.subject.for2020 | 310806 Plant physiology | en |
local.subject.seo2020 | 260199 Environmentally sustainable plant production not elsewhere classified | en |
Appears in Collections: | Journal Article School of Environmental and Rural Science |
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