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https://hdl.handle.net/1959.11/8046
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mitic, N | en |
dc.contributor.author | Valizadeh, M | en |
dc.contributor.author | Leung, EWW | en |
dc.contributor.author | de Jersey, J | en |
dc.contributor.author | Hamilton, S | en |
dc.contributor.author | Hume, DA | en |
dc.contributor.author | Cassady, Alan | en |
dc.contributor.author | Schenk, G | en |
dc.date.accessioned | 2011-07-15T10:09:00Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Archives of Biochemistry and Biophysics, 439(2), p. 154-164 | en |
dc.identifier.issn | 1096-0384 | en |
dc.identifier.issn | 0003-9861 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/8046 | - |
dc.description.abstract | Proteolytic cleavage in an exposed loop of human tartrate-resistant acid phosphatase (TRAcP) with trypsin leads to a significant increase in activity. At each pH value between 3.25 and 8.0 the cleaved enzyme is more active. Substrate specificity is also influenced by proteolysis. Only the cleaved form is able to hydrolyze unactivated substrates efficiently, and at pH >6 cleaved TRAcP acquires a marked preference for ATP. The cleaved enzyme also has altered sensitivity to inhibitors. Interestingly, the magnitude and mode of inhibition by fluoride depends not only on the proteolytic state but also pH. The combined kinetic data imply a role of the loop residue D158 in catalysis in the cleaved enzyme. Notably, at low pH this residue may act as a proton donor for the leaving group. In this respect the mechanism of cleaved TRAcP resembles that of sweet potato purple acid phosphatase. | en |
dc.language | en | en |
dc.publisher | Academic Press | en |
dc.relation.ispartof | Archives of Biochemistry and Biophysics | en |
dc.title | Human tartrate-resistant acid phosphatase becomes an effective ATPase upon proteolytic activation | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1016/j.abb.2005.05.013 | en |
dc.subject.keywords | Enzymes | en |
local.contributor.firstname | N | en |
local.contributor.firstname | M | en |
local.contributor.firstname | EWW | en |
local.contributor.firstname | J | en |
local.contributor.firstname | S | en |
local.contributor.firstname | DA | en |
local.contributor.firstname | Alan | en |
local.contributor.firstname | G | en |
local.subject.for2008 | 060107 Enzymes | en |
local.subject.seo2008 | 970106 Expanding Knowledge in the Biological Sciences | en |
local.profile.school | School of Science and Technology | en |
local.profile.email | acassady@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20110715-09486 | en |
local.publisher.place | United States of America | en |
local.format.startpage | 154 | en |
local.format.endpage | 164 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 439 | en |
local.identifier.issue | 2 | en |
local.contributor.lastname | Mitic | en |
local.contributor.lastname | Valizadeh | en |
local.contributor.lastname | Leung | en |
local.contributor.lastname | de Jersey | en |
local.contributor.lastname | Hamilton | en |
local.contributor.lastname | Hume | en |
local.contributor.lastname | Cassady | en |
local.contributor.lastname | Schenk | en |
dc.identifier.staff | une-id:acassady | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:8220 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Human tartrate-resistant acid phosphatase becomes an effective ATPase upon proteolytic activation | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Mitic, N | en |
local.search.author | Valizadeh, M | en |
local.search.author | Leung, EWW | en |
local.search.author | de Jersey, J | en |
local.search.author | Hamilton, S | en |
local.search.author | Hume, DA | en |
local.search.author | Cassady, Alan | en |
local.search.author | Schenk, G | en |
local.uneassociation | Unknown | en |
local.year.published | 2005 | en |
Appears in Collections: | Journal Article |
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