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https://hdl.handle.net/1959.11/8042
Title: | Macrophages overexpressing tartrate-resistant acid phosphatase show altered profile of free radical production and enhanced capacity of bacterial killing | Contributor(s): | Raisanen, SR (author); Alatalo, SL (author); Ylipahkala, H (author); Halleen, JM (author); Cassady, Alan (author); Hume, DA (author); Vaananen, HK (author) | Publication Date: | 2005 | DOI: | 10.1016/j.bbrc.2005.03.133 | Handle Link: | https://hdl.handle.net/1959.11/8042 | Abstract: | Activated macrophages and osteoclasts express high amounts of tartrate-resistant acid phosphatase (TRACP, acp5). TRACP has a binuclear iron center with a redox-active iron that has been shown to catalyze the formation of reactive oxygen species (ROS) by Fenton's reaction. Previous studies suggest that ROS generated by TRACP may participate in degradation of endocytosed bone matrix products in resorbing osteoclasts and degradation of foreign compounds during antigen presentation in activated macrophages. Here we have compared free radical production in macrophages of TRACP overexpressing (TRACP+) and wild-type (WT) mice. TRACP overexpression increased both ROS levels and superoxide production. Nitric oxide production was increased in activated macrophages of WT mice, but not in TRACP+ mice. Macrophages from TRACP+ mice showed increased capacity of bacterial killing. Recombinant TRACP enzyme was capable of bacterial killing in the presence of hydrogen peroxide. These results suggest that TRACP has an important biological function in immune defense system. | Publication Type: | Journal Article | Source of Publication: | Biochemical and Biophysical Research Communications, 331(1), p. 120-126 | Publisher: | Elsevier Inc | Place of Publication: | United Kingdom | ISSN: | 1090-2104 0006-291X |
Fields of Research (FoR) 2008: | 060103 Cell Development, Proliferation and Death | Socio-Economic Objective (SEO) 2008: | 920108 Immune System and Allergy | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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