Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/7496
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dc.contributor.authorMoens, Pierreen
dc.contributor.authorGratton, Enricoen
dc.contributor.authorSalvemini, Iyrrien
dc.date.accessioned2011-05-20T10:34:00Z-
dc.date.issued2011-
dc.identifier.citationMicroscopy Research and Technique, 74(4), p. 377-388en
dc.identifier.issn1097-0029en
dc.identifier.issn1059-910Xen
dc.identifier.urihttps://hdl.handle.net/1959.11/7496-
dc.description.abstractFluorescence correlation spectroscopy (FCS) was developed in 1972 by Magde, Elson and Webb. Photon counting detectors and avalanche photodiodes have become standards in FCS to the point that there is a widespread belief that these detectors are essential to perform FCS experiments, despite the fact that FCS was developed using analog detectors. Spatial and temporal intensity fluctuation correlations using analog detection on a commercial Olympus Fluoview 300 microscope have been reported by Brown et al. (2008). However, each analog instrument has its own idiosyncrasies that need to be understood before using the instrument for FCS. In this work, we explore the capabilities of the Nikon C1, a low-cost confocal microscope, to obtain single point FCS, Raster-scan image correlation spectroscopy (RICS), and Number and Brightness data both in solution and incorporated into the membrane of giant unilamellar vesicles. We show that it is possible to obtain dynamic information about fluorescent molecules from single point FCS, RICS, and Number and Brightness using the Nikon C1. We highlighted the fact that care should be taken in selecting the acquisition parameters to avoid possible artifacts due to the detector noise. However, due to relatively large errors in determining the distribution of digital levels for a given microscope setting, the system is probably only adequate for determining relative brightness within the same image.en
dc.languageenen
dc.publisherJohn Wiley & Sons, Incen
dc.relation.ispartofMicroscopy Research and Techniqueen
dc.titleFluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1)en
dc.typeJournal Articleen
dc.identifier.doi10.1002/jemt.20919en
dcterms.accessRightsGreenen
dc.subject.keywordsBiological Physicsen
local.contributor.firstnamePierreen
local.contributor.firstnameEnricoen
local.contributor.firstnameIyrrien
local.subject.for2008029901 Biological Physicsen
local.subject.seo2008920102 Cancer and Related Disordersen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.emailpmoens@une.edu.auen
local.profile.emailegratton22@yahoo.comen
local.profile.emailisalvem2@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20110223-12087en
local.publisher.placeUnited States of Americaen
local.format.startpage377en
local.format.endpage388en
local.identifier.scopusid84893442576en
local.url.openhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001136en
local.peerreviewedYesen
local.identifier.volume74en
local.identifier.issue4en
local.access.fulltextYesen
local.contributor.lastnameMoensen
local.contributor.lastnameGrattonen
local.contributor.lastnameSalveminien
dc.identifier.staffune-id:pmoensen
dc.identifier.staffune-id:isalvem2en
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:7664en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleFluorescence Correlation Spectroscopy, Raster Image Correlation Spectroscopy, and Number and Brightness on a Commercial Confocal Laser Scanning Microscope with Analog Detectors (Nikon C1)en
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.relation.grantdescriptionNHMRC/568301en
local.search.authorMoens, Pierreen
local.search.authorGratton, Enricoen
local.search.authorSalvemini, Iyrrien
local.uneassociationUnknownen
local.identifier.wosid000288859800012en
local.year.published2011en
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