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Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/7177
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dc.contributor.authorWu, Shubiaoen
dc.contributor.authorRodgers, Nicholasen
dc.contributor.authorChoct, Minganen
dc.date.accessioned2011-04-20T16:11:00Z-
dc.date.issued2011-
dc.identifier.citationApplied and Environmental Microbiology, 77(3), p. 1135-1139en
dc.identifier.issn1098-5336en
dc.identifier.issn0099-2240en
dc.identifier.urihttps://hdl.handle.net/1959.11/7177-
dc.description.abstractWe compared ileal 'Clostridium perfringens' quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying 'C. perfringens' in the intestinal tracts of broiler chickens.en
dc.languageenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofApplied and Environmental Microbiologyen
dc.titleReal-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritisen
dc.typeJournal Articleen
dc.identifier.doi10.1128/AEM.01803-10en
dcterms.accessRightsGolden
dc.subject.keywordsAnimal Protection (Pests and Pathogens)en
local.contributor.firstnameShubiaoen
local.contributor.firstnameNicholasen
local.contributor.firstnameMinganen
local.subject.for2008070205 Animal Protection (Pests and Pathogens)en
local.subject.seo2008830309 Poultryen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailswu3@une.edu.auen
local.profile.emailnrodger2@une.edu.auen
local.profile.emailmchoct@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20110314-15200en
local.publisher.placeUnited States of Americaen
local.format.startpage1135en
local.format.endpage1139en
local.identifier.scopusid79551502291en
local.peerreviewedYesen
local.identifier.volume77en
local.identifier.issue3en
local.access.fulltextYesen
local.contributor.lastnameWuen
local.contributor.lastnameRodgersen
local.contributor.lastnameChocten
dc.identifier.staffune-id:swu3en
dc.identifier.staffune-id:nrodger2en
dc.identifier.staffune-id:mchocten
local.profile.orcid0000-0002-1790-6015en
local.profile.orcid0000-0002-2242-8222en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:7343en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleReal-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritisen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorWu, Shubiaoen
local.search.authorRodgers, Nicholasen
local.search.authorChoct, Minganen
local.uneassociationUnknownen
local.year.published2011en
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