Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/6286
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dc.contributor.authorCoumans-Moens, Joelleen
dc.contributor.authorPoljak, Anneen
dc.contributor.authorRaftery, Mark Jen
dc.contributor.authorBackhouse, Daviden
dc.contributor.authorPereg, Lilyen
dc.date.accessioned2010-07-27T11:23:00Z
dc.date.issued2009en
dc.identifier.citationProteomics, 9(2), p. 335-349en
dc.identifier.issn1615-9861en
dc.identifier.urihttps://hdl.handle.net/1959.11/6286en
dc.description.abstractA proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with 'Thielaviopsis basicola'. Microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-DE analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein expression levels showed four major profiles (constant, upregulated, one slightly downregulated, and one dramatically downregulated). Spots significantly (p<0.05) upregulated were analyzed by LC-MS/MS and identified using MASCOT MS/MS ion search software and associated databases. These proteins included defense and stress related proteins, such as pathogenesis-related proteins and proteins likely to be involved in the oxidative burst, sugar, and nitrogen metabolism as well as amino acid and isoprenoid synthesis. While many of the identified proteins are common components of the defense response of most plants, a proteasome subunit and a protein reported to be induced only in cotton root following 'Meloidogyne incognita' infection were also identified.en
dc.languageenen
dc.publisherWiley-VCH Verlag GmbH & Co KGaAen
dc.relation.ispartofProteomicsen
dc.titleAnalysis of cotton ('Gossypium hirsutum') root proteomes during a compatible interaction with the black root rot fungus 'Thielaviopsis basicola'en
dc.typeJournal Articleen
dc.identifier.doi10.1002/pmic.200800251en
dc.subject.keywordsProteomics and Intermolecular Interactions (excl Medical Proteomics)en
dc.subject.keywordsPlant Biologyen
local.contributor.firstnameJoelleen
local.contributor.firstnameAnneen
local.contributor.firstnameMark Jen
local.contributor.firstnameDaviden
local.contributor.firstnameLilyen
local.subject.for2008060799 Plant Biology not elsewhere classifieden
local.subject.for2008060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)en
local.subject.seo2008829999 Plant Production and Plant Primary Products not elsewhere classifieden
local.profile.schoolSchool of Rural Medicineen
local.profile.schoolChemistryen
local.profile.schoolChemistryen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailjmoensco@une.edu.auen
local.profile.emaildbackhou@une.edu.auen
local.profile.emaillperegge@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20090623-154521en
local.publisher.placeWeinheim, Germanyen
local.format.startpage335en
local.format.endpage349en
local.peerreviewedYesen
local.identifier.volume9en
local.identifier.issue2en
local.contributor.lastnameCoumans-Moensen
local.contributor.lastnamePoljaken
local.contributor.lastnameRafteryen
local.contributor.lastnameBackhouseen
local.contributor.lastnamePeregen
dc.identifier.staffune-id:jmoenscoen
dc.identifier.staffune-id:dbackhouen
dc.identifier.staffune-id:lpereggeen
local.profile.orcid0000-0001-6642-5202en
local.profile.orcid0000-0003-0663-6002en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:6443en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleAnalysis of cotton ('Gossypium hirsutum') root proteomes during a compatible interaction with the black root rot fungus 'Thielaviopsis basicola'en
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.description.statisticsepubsVisitors: 463<br />Views: 523<br />Downloads: 0en
local.search.authorCoumans-Moens, Joelleen
local.search.authorPoljak, Anneen
local.search.authorRaftery, Mark Jen
local.search.authorBackhouse, Daviden
local.search.authorPereg, Lilyen
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