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https://hdl.handle.net/1959.11/5969
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DC Field | Value | Language |
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dc.contributor.author | Thompson, Janene L | en |
dc.contributor.author | Thomas, Philip | en |
dc.contributor.author | Schuller, Kathryn A | en |
dc.date.accessioned | 2010-05-25T16:03:00Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 144(1), p. 86-93 | en |
dc.identifier.issn | 1878-1659 | en |
dc.identifier.issn | 1532-0456 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/5969 | - |
dc.description.abstract | A glutathione peroxidase (GPX) protein was purified approximately 1000-fold from Southern bluefin tuna (Thunnus maccoyii) liver to a final specific activity of 256 μmol NADPH oxidised min−¹ mg−¹ protein. Gel filtration chromatography and denaturing protein gel electrophoresis of the purified preparation indicated that the protein has a native molecular mass of 85 kDa and is most likely a homotetramer with subunits of approximately 24 kDa. The Km values of the purified enzyme for hydrogen peroxide, cumene hydroperoxide, t-butyl hydroperoxide and glutathione were 12, 90, 90 and 5900 μM, respectively. The Km values for cumene hydroperoxide and t-butyl hydroperoxide were approximately 8-fold greater than the Km value for hydrogen peroxide. Thus, the SBT liver GPX has a considerably greater affinity for hydrogen peroxide than for the other two substrates. The pH optimum of the purified enzyme was pH 8.0. Immunoblotting experiments with polyclonal antibodies, raised against a recombinant human GPX, provided further evidence that the purified SBT enzyme is a genuine GPX. | en |
dc.language | en | en |
dc.publisher | Elsevier Inc | en |
dc.relation.ispartof | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | en |
dc.title | Purification and properties of a glutathione peroxidase from Southern bluefin tuna ('Thunnus maccoyii') liver | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1016/j.cbpb.2006.01.011 | en |
dc.subject.keywords | Biological Sciences | en |
local.contributor.firstname | Janene L | en |
local.contributor.firstname | Philip | en |
local.contributor.firstname | Kathryn A | en |
local.subject.for2008 | 069999 Biological Sciences not elsewhere classified | en |
local.subject.seo2008 | 839999 Animal Production and Animal Primary Products not elsewhere classified | en |
local.profile.school | Business Economics and Public Policy | en |
local.profile.email | pthoma22@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20100423-143712 | en |
local.publisher.place | United States of America | en |
local.format.startpage | 86 | en |
local.format.endpage | 93 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 144 | en |
local.identifier.issue | 1 | en |
local.contributor.lastname | Thompson | en |
local.contributor.lastname | Thomas | en |
local.contributor.lastname | Schuller | en |
dc.identifier.staff | une-id:pthoma22 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:6114 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Purification and properties of a glutathione peroxidase from Southern bluefin tuna ('Thunnus maccoyii') liver | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Thompson, Janene L | en |
local.search.author | Thomas, Philip | en |
local.search.author | Schuller, Kathryn A | en |
local.uneassociation | Unknown | en |
local.year.published | 2006 | en |
Appears in Collections: | Journal Article |
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