Harnessing the power of the innate immune system to protect livestock against disease

Abstract

This collection of data is from four experimental trials between 2019 and 2022, investigating the effect of the immune stimulant Amplimune® (NovaVive Inc., Napanee, Ontario, Canada) on immune and physiological responses in Australian beef cattle. The aim was to explore the potential of the immune stimulant to enhance immune responses and protect cattle against a range of pathogens. At CSIRO’s Chiswick Research Station near Armidale, NSW, immune and physiological responses were measured following administration of Amplimune to cattle in vivo (live animal), or cattle immune cells in vitro (in the laboratory). Immune responses were measured using a haematological analyser, enzyme-linked immunosorbent assays, cell culture and RNA extraction, PCR and sequencing. Physiological responses were measured using temperature loggers, weight scales and physical assessment (by a veterinarian). These data (except the sequencing data) were all stored in excel spreadsheets. The sequencing data was stored as zipped fastq files.