Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/5556
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dc.contributor.authorDeacon, L Jen
dc.contributor.authorGrinev, D Ven
dc.contributor.authorCrawford, J Wen
dc.contributor.authorHarris, Jen
dc.contributor.authorRitz, Ken
dc.contributor.authorYoung, Iainen
dc.date.accessioned2010-04-15T10:50:00Z
dc.date.issued2008en
dc.identifier.citationPedosphere, 18(3), p. 284-287en
dc.identifier.issn1002-0160en
dc.identifier.urihttps://hdl.handle.net/1959.11/5556en
dc.description.abstractThere is a need to simultaneously preserve evidence of interactions between the biological community and soil structural properties of a soil in as near an intact (natural) state as possible. Three dehydration techniques were implemented and assessed for their ability to minimise disruption of both biological and physical properties of the same arable soil sample. Dehydration techniques applied until samples were at constant weight were i) air-drying at 20 °C (AD); ii) -80 °C freeze for 24 h, followed by freeze-drying (-80FD); and iii) liquid nitrogen snap freeze, followed by freeze-drying (LNFD) and were compared to a moist control. Physical structure was determined and quantified in three dimensions using X-ray computed tomography and microbial phenotypic community composition was assessed using phospholipid fatty acid (PLFA) profiling. This study confirms that any form of dehydration, when preparing soil for simultaneous biological and physical analysis, will alter the soil physical properties, and cause some change in apparent community structure. Freeze-drying (both the LNFD and −80FD treatments) was found to minimise disruption (when compared to the moist control soil) to both the soil physical properties and the community structure and is a preferable technique to air-drying which markedly alters the size and character of the pore network, as well as the phenotypic profile. The LNFD was the preferred treatment over the -80FD treatment as samples show low variability between replicates and a fast turn-around time between samples. Therefore snap freezing in liquid nitrogen, followed by freeze drying is the most appropriate form of dehydration when two sets of data, both physical and biological, need to be preserved simultaneously from a soil core.en
dc.languageenen
dc.publisherKexue Chubansheen
dc.relation.ispartofPedosphereen
dc.titleSimultaneous Preservation of Soil Structural Properties and Phospholipid Profiles: A Comparison of Three Drying Techniquesen
dc.typeJournal Articleen
dc.identifier.doi10.1016/S1002-0160(08)60018-1en
dc.subject.keywordsSoil Physicsen
dc.subject.keywordsSoil Biologyen
dc.subject.keywordsSoil Sciencesen
dc.subject.keywordsTerrestrial Ecologyen
local.contributor.firstnameL Jen
local.contributor.firstnameD Ven
local.contributor.firstnameJ Wen
local.contributor.firstnameJen
local.contributor.firstnameKen
local.contributor.firstnameIainen
local.subject.for2008050305 Soil Physicsen
local.subject.for2008050399 Soil Sciences not elsewhere classifieden
local.subject.for2008060208 Terrestrial Ecologyen
local.subject.for2008050303 Soil Biologyen
local.subject.seo2008961402 Farmland, Arable Cropland and Permanent Cropland Soilsen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailiyoung4@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20100219-112210en
local.publisher.placeBeijing, Chinaen
local.format.startpage284en
local.format.endpage287en
local.peerreviewedYesen
local.identifier.volume18en
local.identifier.issue3en
local.title.subtitleA Comparison of Three Drying Techniquesen
local.contributor.lastnameDeaconen
local.contributor.lastnameGrineven
local.contributor.lastnameCrawforden
local.contributor.lastnameHarrisen
local.contributor.lastnameRitzen
local.contributor.lastnameYoungen
dc.identifier.staffune-id:iyoung4en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:5688en
dc.identifier.academiclevelAcademicen
local.title.maintitleSimultaneous Preservation of Soil Structural Properties and Phospholipid Profilesen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.description.statisticsepubsVisitors: 632<br />Views: 872<br />Downloads: 0en
local.search.authorDeacon, L Jen
local.search.authorGrinev, D Ven
local.search.authorCrawford, J Wen
local.search.authorHarris, Jen
local.search.authorRitz, Ken
local.search.authorYoung, Iainen
local.uneassociationUnknownen
local.year.published2008en
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