Viability of cotton and canola pollen in the proboscis of Helicoverpa armigera: Implications for spread of transgenes and pollination ecology

Abstract

1. Pollen can be transported thousands of kilometres by insects but its viability after long-distance transport is not known. Knowing the potential for this mechanism to cause outcrossing of transgenes from genetically modified (GM) plants is important for risk assessments.2. The viability of pollen from cotton (Gossypium hirsutum L.) and canola (Brassica napus L.) was determined after placing it on the proboscis of Helicoverpa armigera moths for intervals of up to 32 h. Viability of both cotton and canola pollen declined at a much greater rate when in contact with the moth proboscis. Most was non-viable by 8 h compared with 16 h for control cotton pollen or 32 h for canola pollen.3. There was no significant difference in the rate of decline of pollen viability between the five conventional cotton varieties, or between these and the one GM cotton variety used in these experiments.4. The number of canola pollen grains remaining on the proboscis declined over time. Very few cotton pollen grains were retained on the proboscis.5. The reduction in pollen viability during contact with the proboscis might indicate partial ingestion of the pollen via the proboscis.6. The points above suggest that pollen is unlikely to remain attached or remain viable when carried over large distances by H. armigera. The implications for spread of pollen from transgenic plants and for pollination ecology in general are discussed.