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https://hdl.handle.net/1959.11/3657
Title: | Cryo-sectioning and chemical-fixing ultramicrotomy techniques for imaging rubber latex particle morphology | Contributor(s): | Subramaniam, Nadaraja (author); Simpson, Anne (author); Monteiro, Michael J (author); Shaffer, Olga (author); Fellows, Christopher (author) ; Gilbert, Robert G (author) | Publication Date: | 2004 | DOI: | 10.1002/jemt.20018 | Handle Link: | https://hdl.handle.net/1959.11/3657 | Abstract: | Two methods adapted from biological microscopy are described for a new application in imaging the morphology of rubbery latex particles. In the first method, a drop of latex is frozen in liquid nitrogen, sectioned with a diamond knife and vapour-stained with osmium tetroxide, then viewed by transmission electron microscopy. When applied to latexes made by emulsion polymerization of methyl methacrylate in a natural rubber latex seed, inclusions are clearly visible. A chemical fixation method is then described for imaging the morphology of such rubbery latex particles. Glutaraldehyde is added to the latex, followed by osmium tetroxide. The sample is then dehydrated in ethanol, epoxy resin added, and the sample cured, ultramicrotomed, and imaged with transmission electron microscopy. An inclusion morphology is again clearly seen. | Publication Type: | Journal Article | Source of Publication: | Microscopy Research and Technique, 63(2), p. 111-114 | Publisher: | John Wiley & Sons, Inc | Place of Publication: | United States of America | ISSN: | 1097-0029 1059-910X |
Fields of Research (FoR) 2008: | 030105 Instrumental Methods (excl Immunological and Bioassay Methods) | Socio-Economic Objective (SEO) 2008: | 860608 Rubber and Synthetic Resins | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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