Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/3197
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dc.contributor.authorKrishnan, Kannanen
dc.contributor.authorHolub, Oliveren
dc.contributor.authorGratton, Enricoen
dc.contributor.authorClayton, Andrew H.A.en
dc.contributor.authorCody, Stephenen
dc.contributor.authorMoens, Pierreen
dc.date.accessioned2009-11-20T14:55:00Z-
dc.date.issued2009-
dc.identifier.citationBiophysical Journal, 96(12), p. 5112-5121en
dc.identifier.issn1542-0086en
dc.identifier.issn0006-3495en
dc.identifier.urihttps://hdl.handle.net/1959.11/3197-
dc.description.abstractProfilin, a small cytoskeletal protein, and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P₂] have been implicated in cellular events that alter the cell morphology, such as endocytosis, cell motility, and formation of the cleavage furrow during cytokinesis. Profilin has been shown to interact with PI(4,5)P₂, but the role of this interaction is still poorly understood. Using giant unilamellar vesicles (GUVs) as a simple model of the cell membrane, we investigated the interaction between profilin and PI(4,5)P₂. A number and brightness analysis demonstrated that in the absence of profilin, molar ratios of PI(4,5)P₂ above 4% result in lipid demixing and cluster formations. Furthermore, adding profilin to GUVs made with 1% PI(4,5)P₂ leads to the formation of clusters of both profilin and PI(4,5)P₂. However, due to the self-quenching of the dipyrrometheneboron difluoride-labeled PI(4,5)P₂, we were unable to determine the size of these clusters. Finally, we show that the formation of these clusters results in the destabilization and deformation of the GUV membrane.en
dc.languageenen
dc.publisherElsevier Incen
dc.relation.ispartofBiophysical Journalen
dc.titleProfilin Interaction with Phosphatidylinositol (4,5)-Bisphosphate Destabilizes the Membrane of Giant Unilamellar Vesiclesen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.bpj.2009.03.034en
dc.subject.keywordsBiological Physicsen
dc.subject.keywordsProteomics and Intermolecular Interactions (excl Medical Proteomics)en
local.contributor.firstnameKannanen
local.contributor.firstnameOliveren
local.contributor.firstnameEnricoen
local.contributor.firstnameAndrew H.A.en
local.contributor.firstnameStephenen
local.contributor.firstnamePierreen
local.subject.for2008060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)en
local.subject.for2008029901 Biological Physicsen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailkkrishna@une.edu.auen
local.profile.emailollihol@web.deen
local.profile.emailegratton22@yahoo.comen
local.profile.emailAndrew.Clayton@ludwig.edu.auen
local.profile.emailstephen.cody@med.monash.edu.auen
local.profile.emailpmoens@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20090623-112742en
local.publisher.placeUnited States of Americaen
local.format.startpage5112en
local.format.endpage5121en
local.identifier.scopusid68949103683en
local.peerreviewedYesen
local.identifier.volume96en
local.identifier.issue12en
local.contributor.lastnameKrishnanen
local.contributor.lastnameHoluben
local.contributor.lastnameGrattonen
local.contributor.lastnameClaytonen
local.contributor.lastnameCodyen
local.contributor.lastnameMoensen
dc.identifier.staffune-id:kkrishnaen
dc.identifier.staffune-id:pmoensen
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:3284en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleProfilin Interaction with Phosphatidylinositol (4,5)-Bisphosphate Destabilizes the Membrane of Giant Unilamellar Vesiclesen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.relation.grantdescriptionNHMRC/568301en
local.search.authorKrishnan, Kannanen
local.search.authorHolub, Oliveren
local.search.authorGratton, Enricoen
local.search.authorClayton, Andrew H.A.en
local.search.authorCody, Stephenen
local.search.authorMoens, Pierreen
local.uneassociationUnknownen
local.identifier.wosid000267194600036en
local.year.published2009en
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