Please use this identifier to cite or link to this item:
Title: Understanding bovine trypanosomiasis and trypanotolerance: the promise of functional genomics
Contributor(s): Hill, Emmeline W (author); O'Gorman, Grace M (author); Agaba, Morris (author); Gibson, John Paul  (author); Hanotte, Olivier (author); Kemp, Stephen J (author); Naessens, Jan (author); Coussens, Paul M (author); MacHugh, David E (author)
Publication Date: 2005
DOI: 10.1016/j.vetimm.2005.02.004
Handle Link:
Abstract: African bovine trypanosomiasis, caused by the protozoan parasite Trypanosoma congolense, is endemic throughout sub-Saharan Africa and is a major constraint on livestock production. A promising approach to disease control is to understand and exploit naturally evolved trypanotolerance. We describe the first attempt to investigate the transcriptional response of susceptible Boran ('Bos indicus') cattle to trypanosome infection via a functional genomics approach using a bovine total leukocyte (BOTL) cDNA microarray platform. Four male Boran cattle were experimentally infected with T. congolense and peripheral blood mononuclear cells (PBMC) were collected before infection and 13, 17, 23 and 30 days post-infection (dpi). A reference experimental design was employed using a universal bovine reference RNA pool. Data were normalised to the median of a set of invariant genes (GAPDH) and BRB-Array tools was used to search for statistically significant differentially expressed genes between each time-point. Using a set of 20 microarray hybridisations, we have made a significant contribution to understand the temporal transcriptional response of bovine PBMC in vivo to a controlled trypanosome infection. The greatest changes were evident 13 dpi after parasites were first detected in the blood. Significant differences were observed in clusters of protein kinase C subunits and MHC class I/II related molecules.
Publication Type: Journal Article
Source of Publication: Veterinary Immunology and Immunopathology, 105(4), p. 247-258
Publisher: Elsevier
Place of Publication: Amsterdam, The Netherlands
ISSN: 0165-2427
Field of Research (FOR): 070201 Animal Breeding
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 1786
Views: 1785
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
2 files
File Description SizeFormat 
Show full item record


checked on Dec 3, 2018

Page view(s)

checked on Jan 12, 2019
Google Media

Google ScholarTM



Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.