Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/3006
Title: Understanding bovine trypanosomiasis and trypanotolerance: the promise of functional genomics
Contributor(s): Hill, Emmeline W (author); O'Gorman, Grace M (author); Agaba, Morris (author); Gibson, John Paul  (author); Hanotte, Olivier (author); Kemp, Stephen J (author); Naessens, Jan (author); Coussens, Paul M (author); MacHugh, David E (author)
Publication Date: 2005
DOI: 10.1016/j.vetimm.2005.02.004
Handle Link: https://hdl.handle.net/1959.11/3006
Abstract: African bovine trypanosomiasis, caused by the protozoan parasite Trypanosoma congolense, is endemic throughout sub-Saharan Africa and is a major constraint on livestock production. A promising approach to disease control is to understand and exploit naturally evolved trypanotolerance. We describe the first attempt to investigate the transcriptional response of susceptible Boran ('Bos indicus') cattle to trypanosome infection via a functional genomics approach using a bovine total leukocyte (BOTL) cDNA microarray platform. Four male Boran cattle were experimentally infected with T. congolense and peripheral blood mononuclear cells (PBMC) were collected before infection and 13, 17, 23 and 30 days post-infection (dpi). A reference experimental design was employed using a universal bovine reference RNA pool. Data were normalised to the median of a set of invariant genes (GAPDH) and BRB-Array tools was used to search for statistically significant differentially expressed genes between each time-point. Using a set of 20 microarray hybridisations, we have made a significant contribution to understand the temporal transcriptional response of bovine PBMC in vivo to a controlled trypanosome infection. The greatest changes were evident 13 dpi after parasites were first detected in the blood. Significant differences were observed in clusters of protein kinase C subunits and MHC class I/II related molecules.
Publication Type: Journal Article
Source of Publication: Veterinary Immunology and Immunopathology, 105(4), p. 247-258
Publisher: Elsevier
Place of Publication: Amsterdam, The Netherlands
ISSN: 0165-2427
Field of Research (FOR): 070201 Animal Breeding
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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