Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/20679
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dc.contributor.authorLudeman, Justin Pen
dc.contributor.authorNazari-Robati, Mahdiehen
dc.contributor.authorWilkinson, Brendanen
dc.contributor.authorHuang, Chengen
dc.contributor.authorPayne, Richard Jen
dc.contributor.authorStone, Martin Jen
dc.date.accessioned2017-05-05T16:00:00Z-
dc.date.issued2015-
dc.identifier.citationOrganic & Biomolecular Chemistry, 13(7), p. 2162-2169en
dc.identifier.issn1477-0539en
dc.identifier.issn1477-0520en
dc.identifier.urihttps://hdl.handle.net/1959.11/20679-
dc.description.abstractTyrosine sulfation is a widespread post-translational modification that mediates the interactions of secreted and membrane-associated proteins in such varied biological processes as peptide hormone action, adhesion, blood coagulation, complement activation and regulation of leukocyte trafficking. Due to the heterogeneous nature of tyrosine sulfation, detailed biochemical and biophysical studies of tyrosine sulfation rely on homogenous, synthetic sulfopeptides. Here we describe the synthesis of a fluorescent sulfopeptide (FL-R2D) derived from the chemokine receptor CCR2 and the application of FL-R2D in direct and competitive fluorescence anisotropy assays that enable the efficient measurement of binding affinities between sulfopeptides and their binding proteins. Using these assays, we have found that the binding of the chemokine monocyte chemoattractant protein-1 (MCP-1) to sulfated peptides derived from the chemokine receptor CCR2 is highly dependent on the assay buffer. In particular, phosphate buffer at close to physiological concentrations competes with the receptor sulfopeptide by binding to the sulfopeptide binding pocket on the chemokine surface. Thus, physiological phosphate may modulate the receptor binding selectivity of chemokines.en
dc.languageenen
dc.publisherRoyal Society of Chemistryen
dc.relation.ispartofOrganic & Biomolecular Chemistryen
dc.titlePhosphate modulates receptor sulfotyrosine recognition by the chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2)en
dc.typeJournal Articleen
dc.identifier.doi10.1039/c4ob02262aen
dc.subject.keywordsCheminformatics and Quantitative Structure-Activity Relationshipsen
dc.subject.keywordsProteins and Peptidesen
dc.subject.keywordsBiologically Active Moleculesen
local.contributor.firstnameJustin Pen
local.contributor.firstnameMahdiehen
local.contributor.firstnameBrendanen
local.contributor.firstnameChengen
local.contributor.firstnameRichard Jen
local.contributor.firstnameMartin Jen
local.subject.for2008030406 Proteins and Peptidesen
local.subject.for2008030404 Cheminformatics and Quantitative Structure-Activity Relationshipsen
local.subject.for2008030401 Biologically Active Moleculesen
local.subject.seo2008929999 Health not elsewhere classifieden
local.subject.seo2008970103 Expanding Knowledge in the Chemical Sciencesen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailbwilkin7@une.edu.auen
local.profile.emailmartin.stone@monash.eduen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20170323-111622en
local.publisher.placeUnited Kingdomen
local.format.startpage2162en
local.format.endpage2169en
local.identifier.scopusid84922626424en
local.peerreviewedYesen
local.identifier.volume13en
local.identifier.issue7en
local.contributor.lastnameLudemanen
local.contributor.lastnameNazari-Robatien
local.contributor.lastnameWilkinsonen
local.contributor.lastnameHuangen
local.contributor.lastnamePayneen
local.contributor.lastnameStoneen
dc.identifier.staffune-id:bwilkin7en
local.profile.orcid0000-0003-1866-6540en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:20872en
dc.identifier.academiclevelAcademicen
local.title.maintitlePhosphate modulates receptor sulfotyrosine recognition by the chemokine monocyte chemoattractant protein-1 (MCP-1/CCL2)en
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.relation.grantdescriptionARC/DE130101673en
local.search.authorLudeman, Justin Pen
local.search.authorNazari-Robati, Mahdiehen
local.search.authorWilkinson, Brendanen
local.search.authorHuang, Chengen
local.search.authorPayne, Richard Jen
local.search.authorStone, Martin Jen
local.uneassociationUnknownen
local.year.published2015en
local.subject.for2020340401 Biologically active moleculesen
local.subject.for2020340407 Proteins and peptidesen
local.subject.for2020340404 Cheminformatics and quantitative structure-activity relationshipsen
local.subject.seo2020280105 Expanding knowledge in the chemical sciencesen
local.subject.seo2020280102 Expanding knowledge in the biological sciencesen
local.codeupdate.date2022-02-13T16:33:53.185en
local.codeupdate.epersonbwilkin7@une.edu.auen
local.codeupdate.finalisedtrueen
local.original.for2020340404 Cheminformatics and quantitative structure-activity relationshipsen
local.original.for2020340401 Biologically active moleculesen
local.original.for2020340407 Proteins and peptidesen
local.original.seo2020280105 Expanding knowledge in the chemical sciencesen
local.original.seo2020280102 Expanding knowledge in the biological sciencesen
local.original.seo2020undefineden
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