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Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/19091
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dc.contributor.authorZhao, Xingmeien
dc.contributor.authorWu, Shubiaoen
dc.contributor.authorWang, Yixueen
dc.contributor.authorCui, Guimeien
dc.contributor.authorWang, Xiaoqingen
dc.contributor.authorDu, Jianzhongen
dc.contributor.authorWang, Xiaolien
dc.contributor.authorShang, Yongjinen
dc.contributor.authorSun, Yien
dc.date.accessioned2016-06-01T09:52:00Z-
dc.date.issued2014-
dc.identifier.citationShengwu Jishu Tongbao, 2014(4), p. 77-82en
dc.identifier.issn1002-5464en
dc.identifier.urihttps://hdl.handle.net/1959.11/19091-
dc.description.abstract采用花青素调控因子C1/Bperu分别与玉米胚特异性启动子Glb1和组成型启动子CaMV35S构建成植物转化载体pGlb1CB和p35SCB，并利用基因枪转化方法，将重组表达载体转入玉米幼胚。显微观察结果证实，这两个载体均能在玉米幼胚细胞中瞬时表达。用花青素作为标记基因不仅可以在一定程度上减少公众对转基因生物安全性方面的担忧，而且可以帮助直观地从转化当代和后代种子中通过颜色标记筛选到转化籽粒，从而可以大大简化筛选程序，提高效率，节约检测成本。en
dc.languagezhen
dc.publisherZhongguo Nongye Kexueyuanen
dc.relation.ispartofShengwu Jishu Tongbaoen
dc.title花青素合成调控转录基因作为直观标记的载体构建及其在玉米幼胚中的瞬时表达en
dc.typeJournal Articleen
dc.subject.keywordsCrop and Pasture Biochemistry and Physiologyen
dc.subject.keywordsPlant Cell and Molecular Biologyen
local.contributor.firstnameXingmeien
local.contributor.firstnameShubiaoen
local.contributor.firstnameYixueen
local.contributor.firstnameGuimeien
local.contributor.firstnameXiaoqingen
local.contributor.firstnameJianzhongen
local.contributor.firstnameXiaolien
local.contributor.firstnameYongjinen
local.contributor.firstnameYien
local.subject.for2008060702 Plant Cell and Molecular Biologyen
local.subject.for2008070303 Crop and Pasture Biochemistry and Physiologyen
local.subject.seo2008820401 Maizeen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailswu3@une.edu.auen
local.output.categoryC2en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20160510-144248en
local.publisher.placeChinaen
local.format.startpage77en
local.format.endpage82en
local.identifier.volume2014en
local.identifier.issue4en
local.contributor.lastnameZhaoen
local.contributor.lastnameWuen
local.contributor.lastnameWangen
local.contributor.lastnameCuien
local.contributor.lastnameWangen
local.contributor.lastnameDuen
local.contributor.lastnameWangen
local.contributor.lastnameShangen
local.contributor.lastnameSunen
dc.title.translatedThe Vector Construction of Anthocyanins as a Visual Marker Gene and Its Transient Expression in Maize Immature Embryosen
dc.identifier.staffune-id:swu3en
local.booktitle.translatedBioTechnology Bulletinen
local.profile.orcid0000-0002-1790-6015en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:19289en
dc.identifier.academiclevelAcademicen
local.abstract.englishAnthocyanin regulatory factor C1/Bperu genes are constructed respectively with a maize embryo specific promoter GLB1 and a constitutive promoter CaMV35S into plant transformation vectors pGlb1CB and p35SCB, and the recombinant expression vectors were introduced into maize embryos by particle bombardment. Microscopic observation confirmed that the two vectors were actively expressed in maize immature embryo cells. The use of anthocyanins as a marker gene not only could reduce the safety concerns from the public over GMO substantially, but could also be used as a visual indicator for the selection of transgenic plants, which could greatly simplify the screening procedures, improve the efficiency and reduce the costs.en
local.title.maintitle花青素合成调控转录基因作为直观标记的载体构建及其在玉米幼胚中的瞬时表达en
local.output.categorydescriptionC2 Non-Refereed Article in a Scholarly Journalen
local.relation.urlhttp://d.wanfangdata.com.cn/Periodical/swjstb201404013en
local.relation.urlhttp://biotech.caas.cn/CN/Y2014/V0/I4/77en
local.search.authorZhao, Xingmeien
local.search.authorWu, Shubiaoen
local.search.authorWang, Yixueen
local.search.authorCui, Guimeien
local.search.authorWang, Xiaoqingen
local.search.authorDu, Jianzhongen
local.search.authorWang, Xiaolien
local.search.authorShang, Yongjinen
local.search.authorSun, Yien
local.uneassociationUnknownen
local.atsiresearchNoen
local.sensitive.culturalNoen
local.year.published2014-
local.subject.for2020310803 Plant cell and molecular biologyen
local.subject.for2020300404 Crop and pasture biochemistry and physiologyen
local.subject.seo2020260306 Maizeen
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